Proteomic Differences in the Milk Fat Globule Membrane (MGFM) Between High and Low Milk Yielding Sahiwal Cows

Author: Mahanthesh M.T., Sudarshan Kumar, Jagjiwan Ram, Rohit Kumar, Preethi Bharadwaj, Sumit Singh and A.K. Mohanty

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Abstract

In the present study we have studied the proteomic differences in the milk fat globule membranes of high versus low milk yielding Sahiwal cows (n=12 each). The proteins extracted from milk cream fat were subjected to trypsin digestion and peptides were labelled with Tandem Mass Tag (TMT) and identified quantitatively by LC- MS/MS. Identification using the Mascot search engine revealed 1716 proteins, out of which 439 proteins were differentially expressed (DEPs).Separated the down-regulated and up-regulated proteins based on the protein fold change data obtained from the LC- MS/MS protein analysis. We found 154 up-regulated Proteins and 285 down-regulated Proteins and further selected best 12 up-regulated proteins in low yielders sahiwal cattle and 12 down-regulated proteins from high milk producing sahiwal cattle. The DEPs were further subjected to gene ontology and pathway analysis using Cytoscape plug-in tool that showed involvement of proteins in catalytic activities, binding, enzyme regulatory activities, response to wounding and the acute inflammatory response, endopeptidase inhibitor activity and apoptosis. Proteome guided genomic selection gives accurate signature protein biomarkers for high versus low milk yielding Sahiwal cattle.

Keywords

Proteomics, Milk fat globule membrane, Sahiwal cattle, Differentially expressed proteins, LCMS

Conclusion

In present study we show those 12 up-regulated proteins in high yielders and 12 down-regulated proteins in high yielders of Sahiwal cattle. Therefore, these differentially expressed proteins in high versus low milk yielding Sahiwal cattle may be considered as protein biomarkers for differentiating between high versus low milk yielding Sahiwal cattle.

References

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How to cite this article

Mahanthesh M.T., Sudarshan Kumar, Jagjiwan Ram, Rohit Kumar, Preethi Bharadwaj, Sumit Singh and A.K. Mohanty (2023). Proteomic Differences in the Milk Fat Globule Membrane (MGFM) Between High and Low Milk Yielding Sahiwal Cows. Biological Forum – An International Journal, 15(10): 1274-1281.