Purification and Characterization of Novel Alkaline Serine Protease from Bacillus firmus BAAP-43 isolated from Tannery Industries Soil

Author: Anusuya Balan and Venkatachalam Palanisamy

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Abstract

Enzymes are well-known biocatalysts that may economically expedite and facilitate operations in a variety of sectors, including the detergent, food, pharmaceuticals, and biotechnology industries, by catalysing chemical reactions. An extracellular alkaline serine protease from Bacillus firmus BAAP-43 was purified by ammonium sulfate precipitation, DEAE- Cellulose and followed by Sephadex G-100. Overall yield was 18.7% and molecular weight of the purified enzyme was 30.2 kDa. pH 9.5, temperature 45°C and 1% NaCl showed maximum enzyme activity. Stability of the enzyme was pH 8.0 to 9.5 and temperature upto 50°C. Enzyme was completely inhibited by PMSF and it is an alkaline serine protease. Ca2+ increased the activity of the enzyme and surfactants SDS were slightly inhibited enzyme activity. Among the various organic solvents such as acetone and methanol enhanced the activity. The enzyme showed compatibility with commercial detergents Surf excel improved the cleaning activity. Enzyme showed the high specificity of casein. This study may be the first on the extracellular alkaline serine protease from Bacillus firmus BAAP-43. Formulation of detergents is incessantly developed to improve the cleaning effectiveness and to adapt the fulfil market demands. Thus, it can be considered a potentially powerful detergent agent.

Keywords

Purification, Characterization, Extracellular alkaline serine protease, Bacillus firmus BAAP-43

Conclusion

Bacillus species producing alkaline proteases have significant commercial value because of their wide range of biochemical uses in industries like food, medicine, detergents, silver recovery, and waste management. These properties make alkaline proteases as the best and suitable candidate for industrial applications employing higher temperatures along with shorter reaction times and low risk of contamination. The present study was focused on characterization of alkaline serine protease from Bacillus firmus BAAP-43. The purified alkaline serine protease has high stability with wide range of pH, temperature, NaCl, metal ions, organic solvents, surfactants, protein inhibitors and detergents. The present investigation showed that the enzyme produced by Bacillus firmus BAAP-43 is suitable for commercial exploitation as a detergent agent in detergent industries. It could be further explored for other applications also in many industries.

References

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How to cite this article

Anusuya Balan and Venkatachalam Palanisamy (2023). Purification and Characterization of Novel Alkaline Serine Protease from Bacillus firmus BAAP-43 isolated from Tannery Industries Soil. Biological Forum – An International Journal, 15(5): 87-92.