Quantification of Bioactive Compounds in Piper Betle Leaf extract by Gas Chromatography Mass Spectrometry (GC-MS) Technique

Author: P. Geetha*, V. Sathiamoorthy and M. Balakrishnan

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Abstract

Piper betle is a Piperaceae family scented perennial creeper. The leaves are high in bioactive compounds such as phenol, a compound with anti-tumor, anti-mutagenic, and immune modulatory activities. There are so many bioactive compounds present in betel leaves. To identify the major compound particularly the varieties grown Tamil Nadu is the major concern of the study. The aim of this study is to evaluate the bioactive compound in Piper betle leaves extract using Gas Chromatography Mass Spectrometry (GC-MS). Over 100 compounds were found in the GC-MS results, with 19 of them having a probability of greater than 30.The compound phentermine shows highest peak at retention time of 9.346 mins followed by Hexadecanoic acid, Tetradecanoic acid, Eugenol, Dodecanoic acid. Hexadecanoic acid shows the highest area% about 26.665% which indicate highest composition of hexadecanoic acid present in the betel leaf extract. The result revealed that the compound in betel leaf extract possess the medi

Keywords

Piper betle leaves, Gas Chromatography Mass Spectroscopy, Retention Time and Area.

Conclusion

The compounds identified in the Piper betle leaf extract most have medicinal properties and some have antibacterial activities. Mostly, betel leaf extract consists of fatty acid compounds and also contains phenols, terpenes, amide groups and esters. The extract contains both saturated and unsaturated fatty acid. The GC-MS results revealed phentermine and hexadecanoic acid were found in higher concentrations. Phentermine showed the highest peak at a retention time of 9.346 minutes, whereas hexadecanoic acid recorded the highest percentage area of 26.665%. The water solvent extraction mostly extracts fatty acids from the Piper betle leaf extract than the other compounds. Hence, it can be used for further processing like encapsulation.

References

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How to cite this article

P. Geetha, V. Sathiamoorthy and M. Balakrishnan (2022). Quantification of Bioactive Compounds in Piper Betle Leaf Extract by Gas Chromatography Mass Spectrometry (GC-MS) Technique. Biological Forum – An International Journal, 14(1): 1199-1203.