Quinestrol, a synthetic estrogen, has been shown to induce oxidative stress and alter biochemical functions in vital organs such as the liver and kidneys, in addition to its effects on reproductive tissues. This study evaluates the impact of quinestrol (2 mg/kg body weight) after 10 days of exposure on biochemical parameters, key antioxidative enzymes and lipid peroxidation levels in male albino rats. A total of 40 adult male rats were divided into two groups: control (n=20) and quinestrol-treated (n=20). Reduction in liver and kidney weights indicates organ atrophy and possible toxicity. Biochemical analysis revealed a significant decrease in total protein, total lipid, cholesterol and phospholipid levels in liver and kidney homogenates, suggesting metabolic disturbances and impaired organ function. Additionally activity of key antioxidant enzymes, including glutathione peroxidase, glutathione-S-transferase, catalase, glutathione reductase and superoxide dismutase decreased in all the reproductive organs, leading to a compromised antioxidant defense system. Increased lipid peroxidation confirmed heightened oxidative stress, which may contribute to reproductive toxicity, impaired spermatogenesis and reduced sperm quality. These findings emphasize the detrimental impact of quinestrol on vital organ integrity and male reproductive health, highlighting the need for further investigation into its toxicological effects

Antioxidant enzymes, biochemical functions, quinestrol, vital organs

In short, oral contraceptives can affect kidney and liver function through multiple pathways, including altering biochemical markers and inducing oxidative stress, as seen with quinestrol if consumed in doses higher than the prescribed one. While some components may have benefits, combinations can lead to adverse effects which calls for further investigations to develop safer options in field of oral contraceptives

Mohit Pun and Nisha Vashishat (2025). Quinestrol-induced Oxidative Stress: Implications for Vital Organ Function and Male Reproductive Health. Biological Forum, 17(5a): 24-30