Traditional siddha practice is a medical skill that is distinctive in both composition and mechanism. The main reason for the efficacy is that siddha formulations are composed of potentially bioactive plants. The major purpose of the current investigation is to evaluate the safety profile of the siddha formulation Athiyadhi kashayam (AK) in both short-term (acute) and long-term (sub-acute) toxicity tests in accordance with the rules established by the OECD. During the course of this inquiry, each of the test animals received a single dosage of 2,000 mg/kg by oral administration, and they were monitored for a period of one week. During the course of the subacute study, experimental rats were given multiple doses of the investigational medication AK (200 and 400 mg/kg/day) over the course of 28 days. The findings of the acute trial revealed that there was no discernible shift in clinical observation after 14 days of close monitoring and observation in rats that had been given the drug. In the subacute investigation, there was no statistically significant difference seen between the animals in the AK treatment group and the animals in the control group in terms of body weight, food/water consumption, haematological observations, or serological findings. Outcome of our study revealed evidence-based data on the safety of the siddha drug AK and proved its safe therapeutic usage on humans

Siddha, Toxicity Study, Athiyadhi Kashayam, Acute, Subacute, Hematology, Serology

Siddha, an ancient medical system, is continually providing credible leads for the discovery of therapeutically effective bioactive components from herbs. Therefore, traditional knowledge supported by modern science is necessary to extrapolate the safety profile of the siddha formulations. Acute toxicity testing showed that a dosage of 2000 mg/kg of the trial medication KV had no influence on the animals' normal physiological functioning or behavioural. There were no reported fatalities over the 14-day duration of the trial. Sub-acute toxicity study findings support no obvious changes in body weight, food intake, water consumption, haematological, or serological profiles of the rats. The current study's findings supplied evidence-based data on the safety nature of the siddha medicine Athiyadhi kashayam, and they also gave justification for the secure clinical use on people with previous suggested validations

C. Meenakshi and G. Bharathkumar (2023). Safety Assessment of Siddha Formulation Athiyadhi kashayam by Short and Long-term Toxicity Studies in Wistar Rats. Biological Forum – An International Journal, 15(3a): 28-33