This study examines seasonal variation in Actin-Beta 2 gene expression in Catla catla (catla fish) from Kolar Reservoir, Bhopal District, Madhya Pradesh. Understanding how gene expression fluctuates with the seasons is crucial for insights into fish growth, health, and aquaculture practices. A total of 30 specimens were collected during winter, summer, and monsoon to assess the impact of changing environmental conditions on gene expression levels. Quantitative PCR (qPCR) was employed to analyze gene expression, revealing significant differences across the seasons. Notably, Catla catla showed markedly higher Actin-Beta 2 expression during monsoon compared to winter (P < 0.05). This increase is likely due to favorable conditions like enhanced food availability and optimal water temperatures during the monsoon period. In contrast, no significant differences were found between winter and summer, suggesting that these seasons may impose similar physiological stresses. The findings highlight the critical role environmental factors play in influencing muscle development in Catla catla. These insights are essential for fisheries management and optimizing aquaculture practices. By understanding how seasonal changes affect gene expression, this research can guide selective breeding strategies focused on improving growth rates and resilience in catla fish. Overall, this study provides valuable information on the physiological adaptations of Catla catla, with implications for enhancing aquaculture sustainability and productivity in freshwater ecosystems. Future research should further explore the underlying molecular mechanisms associated with these seasonal changes and their effects on fish health and development

Catla catla, Actin-Beta 2 gene, seasonal variation, gene expression, Kolar Reservoir, aquaculture, freshwater ecosystems

This study highlights the significant seasonal variation in Actin-Beta 2 gene expression in Catla catla from Kolar Reservoir, revealing how environmental factors influence muscle development. The findings underscore the importance of understanding these patterns for effective fisheries management and enhancing aquaculture practices. By identifying the impacts of different seasons on gene expression, we can optimize breeding strategies to improve growth rates and resilience in Catla catla. Future research is needed to explore the molecular mechanisms behind these changes and their implications for fish health and productivity

Pratima Mishra, Mukesh Dixit and Kalpana Dave (2025). Seasonal Variation in Actin-Beta 2 Gene Expression in Catla catla from Kolar Reservoir, Bhopal District, Madhya Pradesh. Biological Forum, 17(5): 140-143