Simple, Rapid, and a Green Derivative ATR-FTIR spectroscopic method for the analysis of Atenolol Loaded Microspheres and its tablet formulations

Author: Annapoorani Arjunan, Ruby S., Kumar M. and Bhuvaneswari R.

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Abstract

Developing a non-destructive analytical method to quantify the polymer based analyte is an herculean task. Since there is lacuna in the analysis of drugs in presence of interfering formulation excipients we proposed ATR-FTIR spectroscopic method for the qualitative and quantitative estimation of atenolol in its polymer based microsphere formulations. The current research describes the utility of a validated, simple, rapid, easy-to-implement, and solvent free and green Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy method for the quantitative analysis of Atenolol loaded lab-made microspheres and its marketed tablets. This ATR-FTIR spectroscopic analysis is based on the measurement of the absorbance of infrared bands corresponding to the C=O stretching for amide (–CONH2) in the range of 1650-1700cm-1 of the carbonyl amide moiety of atenolol. The wave number was selected by derivatisation of the absorbance spectrum to its first, second and third derivative spectrum. The statistical results were compared and correlated with other analytical methods for the quantification of Atenolol. The polymer excipients of the atenolol loaded microsphere and in the commercial tablet preparation did not interfere with the active drug. The linearity was found in the range 0.1 – 1.0 w/w % with r = 0.9821. Precision of the method was assessed by the repeated analysis of Atenolol by inter and inter and Intraday analysis. The results retrieved showed a small standard and relative standard deviation values. The high percentage of recovery of Atenolol in microspheres, and its marketed tablets (99.96, 99.92 and 100.03%w/w) demonstrate the compliance of the accuracy study limits as per the ICH guidelines. The limit of detection (LOD) and limit of quantification (LOQ) values (0.0528 and 0.1599 w/w %, respectively) indicated the high sensitivity of the method. Thus, the developed ATR-FTIR spectroscopic method showed high accuracy and precision, is considered as nondestructive, solvent free, green, low cost and rapid, and can be applied easily for the pharmaceutical quantitative determination of atenolol in their polymer based and tablet formulations.

Keywords

Atenolol, ATR-FTIR spectroscopy, Derivative spectroscopy, Analysis

Conclusion

Fourier Transform Infrared Spectroscopy is widely recognized technique has been used to identify several compounds, such as pharmaceuticals, cosmetics and foods, but requires expensive equipments and mathematical pretreatments. The quantization of Atenolol through infrared spectroscopy accomplishes with the requirements of specificity, precision, and accuracy in order to be used as a method for the quality control of polymer based dosage forms and in pharmaceuticals. The method has been evaluated for linearity, accuracy, precision and ruggedness in order to ascertain the suitability of the analytical method. The method was applied to marketed samples. It has been proved that the method was selective and linear between the concentrations 10 - 70 μg and correlation coefficient value was found to be 0.9989. The developed method was found to be precise as the % RSD value for repeatability and intermediate precision were 0.1783 and 0.1688, which were less than 2.0%. The percentage recovery was found to be 99.76 ± 0.185. The method is very simple, rapid and economic nature, which makes it especially suitable for routine quality control work. We conclude that Our work was aimed to focus on the implementation of sustainable chemistry by replacing conventional analytical methods developed with the aid of hazardous solvents with our no-solvent green technique without hindering method performance. The future scope of our work is to perform in vitro and in vivo real time dissolution analysis to predict the pharmacokinetic behavior of the drug.

References

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How to cite this article

Annapoorani Arjunan, Ruby S., Kumar M., Bhuvaneswari R. (2023). Simple, Rapid, and a Green Derivative ATR-FTIR spectroscopic method for the analysis of Atenolol Loaded Microspheres and its tablet formulations. Biological Forum – An International Journal, 15(5): 1051-1057.