Determination of GPR55 over Expression by Quantitative Real-time PCR as a Biomarker for Skin SCC Detection

Author: Farzaneh Nayeb Habib*, Soheila S. Kordestani* and Mohammad Shirkhoda**

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Abstract

Chronic wounds resemble the wounds arising with skin squamous cell carcinoma (SCC). Since they are cancerous, they should be treated using novel bioactive wound dressing instead of biopsy and related procedure for detection. Using a noninvasive method is necessary for early detection and proper treatment. The aim of this study is to investigate GPR55 over expression by quantitative real-time PCR as a biomarker for early detection of skin squamous cell carcinoma (SCC). In a clinical trial, 10 patients were randomly assigned to treatment and were compared to 10 healthy people in control group. Over expression of GPR55 gene was evaluated by quantitative real-time PCR in both groups. The expression of GPR55 gene was significantly higher in treatment group compared to the control group. The results of the study suggest that the over expression of GPR55 can be utilized as a biomarker for detection of skin SCC.

Keywords

Skin squamous cell carcinoma; over expression; GPR55 expression; RT-PCR

Conclusion

Detection of SCC employing conclusive and non-invasive methods can be very important to early treatment of a cancer patient. It appears that the over expression of GPR55 gene can be an effective biomarker in SCC patients. These results combined with other non-invasive early detection methods could open a new horizon for the detection and consequent treatment of SCC patients.

References

Chronic wounds resemble the wounds arising with skin squamous cell carcinoma (SCC). Since they are cancerous, they should be treated using novel bioactive wound dressing instead of biopsy and related procedure for detection. Using a noninvasive method is necessary for early detection and proper treatment. Cancer can be defined as a failure within the cells' communication network (Simon, 2010). Skin cancer is currently the most common type of human cancer, and of particular concern, its incidence is increasing at an astonishing rate. Epidemiological and molecular data strongly suggest that non-melanoma skin cancers are associated with excessive exposure to the ultraviolet (UV) radiation in sunlight. Although stratospheric ozone blocks UVC(below 280 nm) radiation as well as part of UVB radiation (280-290 nm) from reaching the surface of the earth, UVB (290-315 nm) and UVA (315-400 nm)reach the surface of the earth, and cause DNA damage, inflammation and erythema, sunburn, gene mutat

How to cite this article

Farzaneh Nayeb Habib, Soheila S. Kordestani and Mohammad Shirkhoda (2017). Determination of GPR55 over Expression by Quantitative Real-time PCR as a Biomarker for Skin SCC Detection , Biological Forum – An International Journal 9(2): 258-264.