Effect of Different Physico-chemical Parameters on Adsorption Performance of Molecularly Imprinted Polymer for Removing Aflatoxin M1 from Cow's Milk

Author: Samad Bodbodak*, Javad Hesari*, Seyed Hadi Peighambardoust* and Mehrdad Mahkam**

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Abstract

In the present study, efficiency of novel molecularly imprinted polymer (MIP) as a selective adsorbent in removal of aflatoxin M1 (AFM1) from cow's milk was evaluated. For this purpose, effects of different physico-chemical parameters such as pH and ionic strength of the solution, agitation mode and adsorption behaviour of different adsorbents were determined by high-performance liquid chromatography. Also, the effects of adsorption on chemical composition of milk were evaluated in the terms of protein, fat, lactose and total solids. The removal efficiency of AFM1 increased with a magnetic stirring mode and rise of pH from 3 to 7. Also, it decreased with an increment of ionic strength. The results showed that under optimized conditions (stirring at 120 rpm, solution pH = 7, contact time 100 min at 30°C) the MIPs had the highest AFM1 removal efficiency (91.33%) and enhance its safety without any significant effects on quality of milk.

Keywords

Aflatoxin M1; Molecularly imprinting polymer; Adsorption; Cow's milk, Ionic strength.

Conclusion

Occurrence of AFM1 in dairy products is a global challenge, especially for infants and children. Therefore, all of the possible methods to enhance the safety of dairy products should be investigated. In the present study, we introduced a new adsorbent to detoxify the milk based on coating SSP with MIP layer. The Oxidized and silylated SSP was used as supporting bed for surface coating of MIP. The prepared MIPs had higher capacity and selectivity toward AFM1 in spiked aqueous solution compared to respective NIPs. The optimum pH, ionic strength and agitation mode for AFM1 removal were 7, 0.5-2.5 % and stirring mode, respectively. The MIP I was most effective adsorbent for removal of AFM1 from spiked model solution and milk sample. Also, the amount of protein, fat, lactose and total solid content of milk sample didn't decrease significantly after treating with the MIP adsorption. On the basis of obtained results, it could be concluded that the MIPs can be applied as an efficient adsorbent

References

Milk and dairy products have important nutritional and functional role in the human diet and provides vital constituents for humans of all ages. Nevertheless, some hazards such as chemical and microbial contaminants, natural toxins, parasites, pesticides, and drug residues, color additives, unapproved ingredients may occur in milk and dairy products which have negative effects on human health. One of the serious global concerns of dairy product consumption is their contamination to mycotoxins such as aflatoxin M1 (AFM1) and ochratoxin A. Aflatoxins are natural, highly toxic, immunosuppressive, mutagenic, carcinogenic and teratogenic metabolites of certain fungi strains (Zinedine and Manes, 2009). AFM1 is the principal metabolite of aflatoxin B1 (AFB1) which found especially in milk and other dairy products as a result of feeding animal with AFB1 contaminated feedstuffs (Prandini et al., 2009; Santili et al., 2015). AFM1 contamination of dairy products is a serious health threatening p

How to cite this article

Samad Bodbodak, Javad Hesari*, Seyed Hadi Peighambardoust and Mehrdad Mahkam (2017). Effect of Different Physico-chemical Parameters on Adsorption Performance of Molecularly Imprinted Polymer for Removing Aflatoxin M1 from Cow's Milk , Biological Forum â