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Hydrothermal and Enzymatic Pretreatment of Apple Pomace for Bioethanol Production bySolid-state Fermentation

Author: Karan Surya* and Dinesh Kumar*

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Abstract

Apple pomace (AP) is agro-industrial lignocellulosic waste generated after extraction of juice from fresh apples. The main objective of this study was, pretreatment of apple pomace with reduced energy input and use of pretreated apple pomace in bioethanol production. In this study, dried apple pomace was pretreated using hydrothermal and enzymatic techniques. Combination of both pretreatments produced highest amount of reducing sugars i.e. 43.1 g/100 g dry matter (DM), which is 10.5 times higher than the initial level of reducing sugars (4.1 g/100 g DM) present in apple pomace. Pretreated apple pomace was further used as substrate in solid-state fermentation for ethanol production. Under optimized fermentation conditions using S. cerevisiae with co-culture (isolate APW-12), 17.5 g ethanol/100 g of dried apple pomace was obtained. In conclusion, high amount of reducing sugars were obtained from apple pomace with minimum use of chemicals, water and energy using hydrothermal and enzymatic

Keywords

apple pomace, pretreatment, Saccharomyces cerevisiae, co-culture, solid-state fermentation, ethanol production

Conclusion

The present study reveals that combination of enzymatic and hydrothermal pretreatment increased reducing sugars availibility in apple pomace by 10.5 fold from initial level of reducing sugars. Also, this pretreatment process does not involve use of any chemicals and lasts only for one hour, making it simple and cost effective for use at industrial scale. Furthermore, optimization of solid-state fermentation for ethanol production by using pretreated apple pomace as substrate resulted in 17.5 g ethanol from 100 g of apple pomace.

References

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How to cite this article

Karan Surya and Dinesh Kumar (2018). Hydrothermal and Enzymatic Pretreatment of Apple Pomace for Bioethanol Production by Solid-state Fermentation. Biological Forum – An International Journal, 10(2): 114-120.