Antimicrobial and Antioxidant Potential of Hibiscus Rosa-sinensis L. in Western Himalaya

Author: Sarvjeet Singh, Abha Gupta, Amita Kumari and Rachna Verma

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Abstract

Hibiscus rosa-sinensis L. is a medicinal plant found in western Himalaya. Methanol and ethanol flower extracts of Hibiscus rosa-sinensis were investigated in present study for its antibacterial and antioxidant activities from western Himalaya. Disc diffusion method was used for determining antimicrobial properties of flower extracts of the plant against standard and clinical isolates of pathogenic bacteria. Methanolic and ethanolic extract showed maximum antimicrobial activity against standard isolate of Pseudomonas aeruginosa which was observed as 11.4±1.7mm and 13.60±2.1mm respectively. The ethanolic extract showed more antimicrobial activity than methanolic extract. The antioxidant property was evaluated by free radical scavenging activity by DPPH assay with IC50 value of 19.54. Thus, the medicinal potential of plant by virtue of its antioxidant and antimicrobial activity could be harnessed for drug formulation.

Keywords

Medicinal plants; Antimicrobial activity; Antioxidant activity; DPPH assay; Disc diffusion.

Conclusion

Present study on profiling of antimicrobial and antioxidant properties of Hibiscus rosa-sinensis from Solan, Himachal Pradesh has confirmed medicinal potential of plant. The secondary metabolites of the plant can help in virtue of modern medicines. The flower extract of Hibiscus rosa-sinensis has wide range of biological activities such as antibacterial, anti-cancerous, antifungal, antiviral and anti-inflammatory. Results of antimicrobial activity against bacterial strains showed maximum activity against standard strain of Pseudomonas aeruginosa (13.6±2.07mm) in ethanolic extract at concentration of 200µg/disc. In present study comparison of both methanol and ethanol extract shows wide range of antimicrobial activity against different bacterial strains and suggests that ethanolic extract shows more inhibition then methanolic extract at 200µg/disc. The results of the DPPH assay suggest that Hibiscus rosa-sinensis have antioxidant potential or free radical scavenging activity. T

References

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How to cite this article

Sarvjeet Singh, Abha Gupta, Amita Kumari and Rachna Verma (2019). Antimicrobial and Antioxidant Potential of Hibiscus Rosa-Sinensis L. in Western Himalaya. Biological Forum – An International Journal, 11(1): 35-40.