Isolation and Virulent Evaluation of Ralstonia solanacearum cause the Bacterial Wilt in Chrysanthemum (Chrysanthemum Sp.) from Mekong Delta and Lam Dong Province

Author: Huynh Ngoc Tam, LeUyen Thanh, Trinh Quang Phap, Tran Thanh Tung, Luu Thai Danh and Nguyen Thi Thu Nga

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Abstract

Six isolates of Ralstonia solanacearum causing the bacterial wilt in chrysanthemum (Chrysanthemum sp.) from Lam Dong province and Mekong Delta were isolated and evaluated for infecting to their host. From this result, the isolate of high virulence is selected to serve for identification and further more for research of the disease prevention in the future. This experiment was investigated at the Plant Pathology Laboratory of the Plant Protection Department, College of Agriculture and Applied Biology, Can Tho University from October 2015 to February 2017. The six isolates of R. solanacearum from Ben Tre, Can Tho, Tien Giang, An Giang, Dong Thap, and Lam Dong provinces were used for testing on chrysanthemum that includes BT-176, CT-30, TG-189, AG-57, ĐT-9, LĐ-34. These colonies of those isolates on King's B medium showed with cream white, convex, smooth, and irregular or round, and on triphenyltetrazolium chloride (TZC) medium with pink in the center and fluidal white in the margin. Th

Keywords

Ralstonia solanacearum, chrysanthemum, bacterial wilt, isolation, virulence, incidence, severity

Conclusion

Results of isolation from naturally infected plants of six different provinces showing bacterial wilt revealed that all isolates showed typical morphological growth of R. solanacearumon TZC medium where; colonies were fluidal white with red center. Their colonies developed on King’s B medium were cream white, convex, smooth, irregular or round. The obtained results are in agreement with those obtained by Kelman (1954). This study is also confirmed that bacterial wilt of chrysanthemum was caused by R. solanacearum based on virulence, and DNA sequence analyses. All six isolates of R. solanacearumshowed virulence against chrysanthemum plants that displayed the different value in bacterial wilt incidence and severity at from 6 to 22 days DAI. In which, isolate ĐT-9 was the highest one among the two tests of R. solanacearumafter 22 DAI. Koch’s postulate showed that all six isolates of R. solanacearum were pathogenic on chrysanthemum and that R. solanacearumwere consistently reisola

References

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How to cite this article

Tam, Huynh Ngọc, Thanh, LeUyen, Phap, Trinh Quang, Tung, Tran Thanh, Danh, Luu Thai and Nga, Nguyen Thi Thu (2019). Isolation and Virulent Evaluation of Ralstonia solanacearum cause the Bacterial Wilt in Chrysanthemum (Chrysanthemum Sp.) from Mekong Del