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Process Optimization for Recovery of Zein (A Valuable Maize Protein) From Corn Meal and Corn Gluten Meal using Microbial Enzymatic Hydrolysis

Author: Sampy Duggal and Dinesh Kumar

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Abstract

In present study Bajaura Makka a corn variety of H.P., India was used for preparation of corn meal (CM) and corn gluten meal (CGM). Both CM and CGM were analyzed for total protein, carbohydrate and fat in the initial substrate and used further for enzymatic treatment individually with protease (Bacillus sp.), amylase (Aspergillus oryzae) and lipase (Aspergillus niger). All enzyme treatments with CM and CGM (100mg/ml w/v) were carried out at pH6.0-10.0 and in all treatments the protein, sugar and zein release was optimum at pH 9.0. In protease treated CM and CGM, 0.174 mg and 0.132 mg of protein, 0.006mg and 0.010mg of reducing sugar was determined with spectrophotometric method. Whereas, in protease treated CM & CGM, 5.01% and 4.81% (w/w) zein respectively was detected with HPLC. Treatment of CM and CGM with amylase resulted in 0.780mg and 0.486mg of protein, 0.326 mg and 0.576mg of reducing sugar and 6.47% and 3.80% of zein release respectively. With lipase treatment 0.202mg and 0.240

Keywords

Bajaura Makka, corn meal (CM), corn gluten meal (CGM), protease, amylase, lipase, zein, HPLC

Conclusion

The recovery of zein from CM and CGM was done by treatment with three microbial enzymes (protease, amylase and lipase) individually and the yield of zein recovery increased when treatment was done with mixture of enzymes. Duggal and Kumar 09 The whole optimized process used in the present study is unique and innovative green process and there is no reports available on use of enzymes for zein recovery in the literature. The reported process completely avoids use of organic solvents for the extraction of zein thus making it a completely green extraction process. Further the work is under process to use immobilized enzyme to improve the reusability of enzyme and reduce the cost of treatment process.

References

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How to cite this article

Sampy Duggal and Dinesh Kumar (2016). Process Optimization for Recovery of Zein (A Valuable Maize Protein) From Corn Meal and Corn Gluten Meal using Microbial Enzymatic Hydrolysis , Biological Forum – An International Journal 8(2): 04-09.