Diagnosis of some Common Uropathogens from Patients with Urinary Tract Infection Symptom in Vietnam by PCR Method

Author: Trong Khoa Dao, Quang Trung Dam,Tuyet Mai Vuong, Thi Thu Hong Le, Nam Hai Truong, and Thi Huyen Do*

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Abstract

Urinary tract infections (UTIs) are frequent disease in Vietnam. Timely, sensitive and accurate identification of uropathogens is great value for UTI management. In this study, 66 urine specimens were collected from patients with UTI symptoms in Saint Paul Hospital, Vietnam. By PCR, specific genes of E. coli, E. faecalis, P. aeruginosa, P. mirabilis, K. pneumoniae were amplified from 46 samples (71.2%) and 71.7% were monopathogens. The dominant uropathogens were E. coli (36.4%), E. faecalis (34.8%), P. aeruginosa (18.2%). E. coli was important agent in multi-bacterial infection. Conventional culture detected bacteria in 30.3% samples but had limitation in the detection of Enterococcus, P. aeruginosa. Moreover, of the 35 negative cultured-samples, we also found the bacteria in 25 samples. This finding suggests that PCR is a good method for E. feacalis, P. aeruginosa detection and is the fast, sensitive method for supplementing conventional urine culture to identify uropathogens.

Keywords

E. coli, E. faecalis, K. pneumoniae, PCR, S. saprophyticus, P. aeruginosa, P. mirabilis, uropathogens.

Conclusion

These findings suggest that PCR method is powerful for detection of E. feacalis, P. aeruginosa than culturing method, and almost negative-cultured samples from patients with typical urinary complaints still have an infection. PCR is the fast, sensitive and accurate method maybe supplementing urine culture for identifications of uropathogens.

References

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How to cite this article

Dao, Trong Khoa, Dam, Quang Trung, Vuong, Tuyet Mai, Le, Thi Thu Hong, Truong, Nam Hai and Do, Thi Huyen (2019). Diagnosis of Some Common Uropathogens from Patients with Urinary Tract Infection Symptom in Vietnam by PCR Method. Biological Forum – An Int