Antibacterial and Antifungal Activity of Stevia rebaudiana (Asteraceae) Leaf Extract in vitro Condition

Author: M. Muradashvili*, N. Jabnidze, L. Koiava, R. Dumbadze, K. Memarne, L. Gorgiladze, G. Meparishvili, A. Kalandia and R. Davitadze

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Abstract

In the present communication we studied antimicrobial activity of Stevia plant extract under in vitro condition against plant phytopathogens, which is stored in the culture collection of the Institute of Phytopathology and Biodiversity. The three different extracts of leaves namely, chloroform, acetone and absolute ethyl alcohol were tested against Ralstonia solanacearum, Pseudomonas syringae pv. actinidiae (PSA), Erwinia amylovora bacteria strains and to the fungal plant pathogens: Alternaria alternata, Colletetrichum gloeosporioides and Fusarium moniliforma. The largest zone of inhibition for R. solanacearum KhPe90 strain was measured 15mm with chloroform extract and 18mm by the ethyl alcohol extract, but 5mm has shown acetone. The maximum inhibition zone for chloroform extract against PSA strains were 18mm and 20 mm were observed for ethyl alcohol extract, while for acetone extract has shown 8mm. For test strains of E. amylovora diameter of lyses area was 12mm with action of chloro

Keywords

Stevia extract; Antimicrobial activity; plant pathogen.

Conclusion

In the present communication we studied antimicrobial activity of Stevia plant extract under in vitro condition against plant phytopathogens, which is stored in the culture collection of the Institute of Phytopathology and Biodiversity. The three different extracts of leaves namely, chloroform, acetone and absolute ethyl alcohol were tested against Ralstonia solanacearum, Pseudomonas syringae pv. actinidiae (PSA), Erwinia amylovora bacteria strains and to the fungal plant pathogens: Alternaria alternata, Colletetrichum gloeosporioides and Fusarium moniliforma. The largest zone of inhibition for R. solanacearum KhPe90 strain was measured 15mm with chloroform extract and 18mm by the ethyl alcohol extract, but 5mm has shown acetone. The maximum inhibition zone for chloroform extract against PSA strains were 18mm and 20 mm were observed for ethyl alcohol extract, while for acetone extract has shown 8mm. For test strains of E. amylovora diameter of lyses area was 12mm with action of chloro

References

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How to cite this article

Muradashvili, M., Jabnidze, N., Koiava, L., Dumbadze, R., Memarne, K., Gorgiladze, L., Meparishvili, G., Kalandia, A. and Davitadze, R. (2019). Antibacterial and Antifungal Activity of Stevia rebaudiana (Asteraceae) Leaf Extract in vitro Condition. Bi