A Report on Metal Enhanced, Solvent Tolerant Endoglucanase from Strict Thermophilic Bacillus sp. PW1 and Bacillus sp. PW2 of North West Himalayas

Author: Divyanshi Sharma, Kamal Dev and Anuradha Sourirajan

Download PDF

Abstract

The cellulolytic activity of two thermophilic bacterial strains Bacillus sp. PW1 and Bacillus sp. PW2 isolated from Tattapani hot spring of North Western Himalayas were characterized. Both the strains exhibited extracellular CMCase activity, indicating the endoglucanase potential of the cellulases. There was no detectable cellulase activity of both the strains below 40ºC, indicating thermophilic nature of the cellulase enzymes. Optimum temperature and pH for cellulase activity of both PW1 and PW2 was 80ºC and 8, respectively. Various metal ions enhanced the cellulase activity of the bacterial strains, with Ca2+, and Hg2+ showing the maximum effect. Interestingly, the cellulase of the two bacterial strains was active in the presence of solvents (0.5 % v/v) like Ethanol, n-butanol and cyclohexane, and the denaturant, SDS (0.5% w/v). Glucose and Galactose were best carbon sources, while beef extract and tryptone the best nitrogen sources for the maximum production of cellulase by the tw

Keywords

Endoglucanase, Thermophilic, metal ions, organic solvent tolerance, Tattapani, hot spring, Himachal Pradesh, India.

Conclusion

In our study, we have explored two thermophiles namely Bacillus sp. PW1 and Bacillus sp. PW2 of Tattapani hot spring (Himachal Pradesh, India) for their cellulolytic potential. Extracellular Cellulase activity of the two bacterial strains was analyzed at various pH and temperature. Maximum activity was observed at pH 8 and 80ºC, indicating thermoalkalophilic nature of the cellulase activity of Bacillus sp. PW1 and Bacillus sp. PW2. Production of cellulase by both the strains was best in media containing Glucose and Galactose as carbon sources, and beef Extract, and tryptone as nitrogen sources. Various metal ions enhanced the cellulase activity of both the bacterial strains, with Hg2+ and Ca2+ showing the best activation of activity. The cellulase activity of both strains showed tolerance to a wide range of solvents and detergents like SDS, thereby revealing their ample potential for industrial applications. Thus, the thermozymes of Bacillus sp. PW1 and Bacillus sp. PW2 characterized

References

-

How to cite this article

Sharma, Divyanshi, Dev, Kamal and Sourirajan, Anuradha (2019). A Report on Metal Enhanced, Solvent Tolerant Endoglucanase from Strict Thermophilic Bacillus sp. PW1 and Bacillus sp. PW2 of North West Himalayas. Biological Forum – An International Jour