Effect of Salt Stress and Exogenous Application of Proline on some Antioxidant Enzymes Activity in Barley Cultivars Seedling

Author: Mohammad Behrouzi Varjovi*, Mostafa Valizadeh* and Mohammad Moghaddam Vahed*

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Abstract

In order to investigate the effect of salt stress and exogenous proline treatment on catalase and peroxidase activity, on ten barley cultivars at seedling stage, a factorial experiment on the basis of completely randomized experimental design in three replications was performed using NaCl levels (0, 100 and 200 mM), and three levels of proline (0, 5 and 10 mM). Electrophoretic analyses were performed by using 8% slab polyacrylamide gels. Two antioxidant enzymes including catalase (CAT) and peroxidase (POX) were stained and for each isozymic band the "density × area" scores onto gels were evaluated by MCID software as enzymatic activity. The analysis of variance of data for CAT showed a significant difference for cultivars, salinity levels, salinity × cultivar interaction and salinity × proline × cultivar interaction. For POX, significant differences for cultivars, salinity levels and salinity × cultivar interaction in all isozymes were observed. In addition, there were significant

Keywords

Antioxidant enzymes, Barley, Proline, Salt stress.

Conclusion

In order to investigate the effect of salt stress and exogenous proline treatment on catalase and peroxidase activity, on ten barley cultivars at seedling stage, a factorial experiment on the basis of completely randomized experimental design in three replications was performed using NaCl levels (0, 100 and 200 mM), and three levels of proline (0, 5 and 10 mM). Electrophoretic analyses were performed by using 8% slab polyacrylamide gels. Two antioxidant enzymes including catalase (CAT) and peroxidase (POX) were stained and for each isozymic band the "density × area" scores onto gels were evaluated by MCID software as enzymatic activity. The analysis of variance of data for CAT showed a significant difference for cultivars, salinity levels, salinity × cultivar interaction and salinity × proline × cultivar interaction. For POX, significant differences for cultivars, salinity levels and salinity × cultivar interaction in all isozymes were observed. In addition, there were significant

References

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How to cite this article

Mohammad Behrouzi Varjovi*, Mostafa Valizadeh* and Mohammad Moghaddam Vahed (2016). Effect of Salt Stress and Exogenous Application of Proline on some Antioxidant Enzymes Activity in Barley Cultivars Seedling , Biological Forum – An International Journ