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Comparative study of Rosa damascenes Mill. and R. Gallica micro-propagation

Author: Mohammad Omidi, Abbas Yadollahi and Malihe Eftekhari

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Abstract

Roses are an important commercial cut flower with a wide range of varieties throughout the world. Due to its economic value, this study aimed to optimize the in vitro propagation protocol of three rose cultivars i.e. two (Kashan and Kazanlik) from Rosa damascene Mill. and one (Tuscany superb) from R. gallica by comparing the micro-propagation stages of the musing nodal explants. Using nano-silver in the medium with concentrations of 0, 25, 50 and 75 µmol in a completely randomized design. Result showed that the quality of Kashan cultivar explant increased while it had adverse effect on the other cultivars. The results obviously showed that liquid medium containing 1.5 mg1-1 6-benzylaminopurine (BAP) affected proliferation depending on rose cultivar. Modified Murashige and Skoog (1962) (MS) medium fortified with 2.0 mg l-1 (BAP) and 0.2mg1-1 indole-3-butyric acid (IBA) gave optimal shoot proliferation. Micro shoots from BAP induced cultures could be rooted easily on indole-3-butyric ac

Keywords

Damask rose, R. gallica, micro-propagation, in vitro rooting, and liquid medium

Conclusion

Roses are an important commercial cut flower with a wide range of varieties throughout the world. Due to its economic value, this study aimed to optimize the in vitro propagation protocol of three rose cultivars i.e. two (Kashan and Kazanlik) from Rosa damascene Mill. and one (Tuscany superb) from R. gallica by comparing the micro-propagation stages of the musing nodal explants. Using nano-silver in the medium with concentrations of 0, 25, 50 and 75 µmol in a completely randomized design. Result showed that the quality of Kashan cultivar explant increased while it had adverse effect on the other cultivars. The results obviously showed that liquid medium containing 1.5 mg1-1 6-benzylaminopurine (BAP) affected proliferation depending on rose cultivar. Modified Murashige and Skoog (1962) (MS) medium fortified with 2.0 mg l-1 (BAP) and 0.2mg1-1 indole-3-butyric acid (IBA) gave optimal shoot proliferation. Micro shoots from BAP induced cultures could be rooted easily on indole-3-butyric ac

References

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How to cite this article

Mohammad Omidi, Abbas Yadollahi and Malihe Eftekhari (2016) . Comparative study of Rosa damascenes Mill. and R. Gallica micro-propagation , Biological Forum – An International Journal 8(1): 135-145.