In vito micro propagation of two native Capparis spinosa L. cultivars from Iran

Author: L. Vojodi Mehrabani

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Abstract

Capparis spinosa L. (Caper) is a multipurpose crop that can be used for ornamental, culinary, cosmetics, pharmaceutical and medicinal purposes. As ornamental shrub, caper plant is used for the prevention of soil erosion and help to conserve soil water reserves. Every part of caper has medicinal uses. Due to the great population diversity and difficulties in conventional propagation methods, for the protection of C. spinosa from genetic erosion; a tissue culture method would be a good way for mass propagation of this plant. In vitro propagation of caper was begun with seeds. The highest shoots proliferation was obtained on MS medium supplemented with 3 mgl-1 cytokinin. Varzegan cultivar had the highest shoot and nod number and shoot length. The results showed that Zeatin was quite effective in the proliferation of capper explants. More Shoot multiplication was obtained at the same medium by sub-culturing shoot segment with 2-3 nodes every seven weeks (for two times). Auxin type (IBA

Keywords

Capparis spinosa, BAP, IBA, Propagation.

Conclusion

Due to the mechanical and possibly physiological dormancy, germination percentage and rate in Capparis spinosa is very low. Using cuttings for the propagation bears many more problems due to hard rooting nature of the plant. Tissue culture methods would be accessible propagation were for this high value crop. The tissue culture protocol employed in the present experiment gave us promising results. The overall results revealed that in between the clones studied, there was a meaningful differences considering proliferation and rooting rate. The highest shoots number and length as well as root number was belonged to ?Varzegan¸ cultivar. BAP had mild effect on the proliferation rate, In contrast; Zeatin was a good choice for the proliferation of Capparis spinosa. The highest rooting percent was recorded in MS medium containing 2 mgl-1 NAA. The data obtained clearly emphasize that, tissue culture and using the right hormonal combination can be tried for the multiplication and possibly mass

References

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How to cite this article

L. Vojodi Mehrabani (2016). In vito micro propagation of two native Capparis spinosa L. cultivars from Iran , Biological Forum – An International Journal 8(2): 144-149.