Introgression of Tomato Leaf Curl Virus (ToLCV) Resistant Gene into two Cultivated Tomato (Solanum lycopersicum L.) Varieties through Marker Assisted Backcross Breeding

Author: B.A. Sowjanya*and O. Sridevi

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Abstract

Tomato (Solanum lycopersicum L.) is widely grown in the tropical and subtropical regions of the world but its growth and production is limited because of most of the diseases. Among all the diseases ToLCV is a devastating disease worldwide causing complete yield loss in affected area. So far, resistance to begomoviruses in tomato has been achieved using wild species, and at least six resistance genes have been studied. The complex epidemiological factors associated with this disease, such as broad host range, high rates of virus evolution and the migratory behaviour of whiteflies make it difficult to develop effective long-term management strategies. Therefore, breeding resistance to this viral disease in tomato cultivars is an essential element of a sustainable approach in managing the diseases caused by begomoviruses. The present study was undertaken to introgress Ty-2 conferring resistance to monopartite begomovirus into two cultivated varieties (GPBT-08 and DMT-2) from two donors i

Keywords

tomato, ToLCV, MABB, Ty-2, Foreground selection, Background selection.

Conclusion

The results of the present study, in general, provides evidence of accuracy and reliability for the TG0302 and P1-16 markers to be applied directly to large-scale MABC programmes for the development of high-yielding leaf curl resistant varieties. Within only two backcross generations, at least 90% of the recurrent parents’ genomes were recovered, and leaf curl resistant NILs were developed, demonstrating that introgression of Ty-2 gene with MABC breeding is much faster than that of conventional breeding. For the most part, the developed fragrant NILs showed better yield-related traits than the donor parents CLN2768A and CLN2777H, possessing a similar yield potential with the recurrent parents GPBT-08 and DMT-2. The present study has overall, provided a clear, fast, and yet affordable route to introgressing Ty-2 gene into tomato lines or varieties, and this would benefit researchers especially those with limited resources.

References

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How to cite this article

Sowjanya, B.A. and Sridevi, O. (2021). Introgression of Tomato Leaf Curl Virus (ToLCV) Resistant Gene into two Cultivated Tomato (Solanum lycopersicum L.) Varieties through Marker Assisted Backcross Breeding. Biological Forum – An International Journal