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Statistical Studies on Exploring and Enhancing the Catalytic Potential of Nitrilase from Thermo-tolerant Geobacillus subterraneus from Hot Water Springs of Manikaran

Author: Mamta Sharma, Chandrika Attri and Amit Seth

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Abstract

The importance of thermostable biomolecules has led to an upsurge of research into organisms from thermophilic sites. The present study was aimed to isolate mandelonitrile degrading microbes from hot water springs of Manikaran using indicator plate assay method. Extensive screening resulted in obtaining nitrilase producing thermophile. On the basis of biochemical tests and 16s rDNA gene sequencing it was designated as Geobacillus subterraneus MAC VI. Further, statistical optimization of culture conditions and process parameters was performed. The nitrilase enzyme of this isolate is inducible in nature which is activated in presence of mandelonitrile. The enzyme activity was increased manifold by giving multiple feedings of inducer. The highest nitrilase activity was recorded at 90°C. The enzyme was highly thermostable as it showed activity (0.098 U/mgdcw) at 100°C even after 5h of incubation. This is the first report on a thermophilic Geobacillus subterraneus mandelonitrile degrad

Keywords

Thermophiles, Manikaran, Geobacillus subterraneus, hyperinduction, mandelonitrile, RSM

Conclusion

A new candidate for the production of thermostable nitrilase identified as Geobacillus subterraneus was isolated from hot water springs of Manikaran which was capable of degrading mandelonitrile. Optimization of culture conditions and process parameters enhanced nitrilase production. Thermo stability of the enzyme was an added advantage of this microbe as it was able to withstand 100°C for 5 h and still exhibited appreciable nitrilase activity.

References

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How to cite this article

Mamta Sharma, Chandrika Attri and Amit Seth (2016). Statistical Studies on Exploring and Enhancing the Catalytic Potential of Nitrilase from Thermo-tolerant Geobacillus subterraneus from Hot Water Springs of Manikaran , Biological Forum – An Internati