Marker Assisted Selection and it’s use against Genetic Improvement of Biotic and Abiotic Stress Tolerance in Rice (Oryza sativa L.)

Author: Venkanolla Sruthi, Sanjeev Kumar, Kummethi Lekhasree, D.V. Sravani and G. Rajitha

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Abstract

Improving of crop in terms of agronomic characters, yield and also tolerance to stress for feeding the growing population. All though this can be achieved by the conventional breeding but it is time consuming, high cost and presence of linkage drag. To overcome this many researchers started using the molecular techniques like molecular markers. This are tightly linked to the trait of interest which overcomes linkage drag and it can identified during seedling stage only. In this review we discuss about the MAS and its procedure, applications in resistance cultivar development, genetic diversity study and for improvement of quality of crop. How Marker assisted back crossing (MABC) help in improving the cultivars for abiotic and biotic stress tolerance by reducing number of back crossing. All most 70 to 80% yield loss in rice is due to this stress which can be overcome by using the molecular markers (SSR, STS, SNP’s etc), Which are tightly linked to the resistance genes like Pi9(blast)

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Conclusion

For breeding of crop’s the conventional breeding is not that convenient and it is time taken process which may not help to meet the demand of population, so using of the molecular marker for improving the agronomic, biotic and abiotic characters, also for the major genes or quantitative genes which are depend on environment conditions MAS is best method as marker are not affected by the climate conditions. MAS used for Assaying genetic diversity, purity of cultivars, for selection of parental lines, mainly MABS and gene pyramiding help in transferring the gene to elite cultivars, for the abiotic and abiotic stress tolerance MABC and gene pyramiding help to produce the resistance cultivars. Sub1A gene are cloned to susceptible cultivar, for drought linked QTL’s are highly tolerant, Saltol is R gene for salinity tolerance, For blast Pi9 are most tolerant gene, Bacterial blight mostly combination of gene like X, Xa5, Xa216 are high tolerance then single gene in cultivar this transfer

References

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How to cite this article

Sruthi, V., Lekhasree, K., Sravani, D.V., Rajitha, G. (2021). Marker Assisted Selection and it’s use against Genetic Improvement of Biotic and Abiotic Stress Tolerance in Rice (Oryza sativa L.). Biological Forum – An International Journal, 13(1): 24