Cotton Bed: An Eco friendly Tool for Eliminating Agrobacterium tumefaciens in Sugarcane Transformation

Author: Niraj Nath Tiwari and Sujeet Pratap Singh

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Abstract

Agrobacterium tumefaciens mediated genetic transformation and surface sterilization of explants with antibiotic is the key stage of genetic transformation process. Mostly it’s done, biotic stresses by Agrobacterium and abiotic stresses by antibiotics that is causes and minimize transformation effectiveness due to necrosis and phytotoxicity of explants cells. This study was conducted to examine the effects of cotton bed over antibiotic treatment on Agrobacterium mediate genetic transformation in sugarcane. Agrobacterium strains LBA4404 were used for the infection of leaf disc explants of sugarcane somatic embryogenic calli. Bacterial removal from infected somatic embryogenic calli explants are always essential and a critical stage for survival of the plantlets. In this experiment, cefotaxime 500 mg/l was used for the washing of infected explants and removal of overgrowth of A. tumefaciens. At the end of the experiments, explants survival efficiency were 13%. Necrosis and phytotoxicit

Keywords

Sugarcane; Agrobacterium tumefaciens; Cotton bed, Embryogenic calli, Genetic transformation

Conclusion

Agrobacterium tumefaciens mediated genetic transformation and surface sterilization of explants with antibiotic is the key stage of genetic transformation process. Mostly it’s done, biotic stresses by Agrobacterium and abiotic stresses by antibiotics that is causes and minimize transformation effectiveness due to necrosis and phytotoxicity of explants cells. This study was conducted to examine the effects of cotton bed over antibiotic treatment on Agrobacterium mediate genetic transformation in sugarcane. Agrobacterium strains LBA4404 were used for the infection of leaf disc explants of sugarcane somatic embryogenic calli. Bacterial removal from infected somatic embryogenic calli explants are always essential and a critical stage for survival of the plantlets. In this experiment, cefotaxime 500 mg/l was used for the washing of infected explants and removal of overgrowth of A. tumefaciens. At the end of the experiments, explants survival efficiency were 13%. Necrosis and phytotoxicit

References

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How to cite this article

Tiwari, N.N. and Singh, S.P. (2021). Cotton Bed: An Eco friendly Tool for Eliminating Agrobacterium tumefaciens in Sugarcane Transformation. Biological Forum – An International Journal, 13(1): 293-298