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MAT vector: a system for production and selection of marker free transgenic plants- A survey and summary

Author: Zouhreh Poudine*, Atefeh Galavi**, Mehrdad Asadian*** and Hassan Shahgholi****

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Abstract

One of the most important transformation methods is the oncogenes (ipt, rol, iaaM/H ) of Agrobacterium which are used as a selectable markers. In MAT (Multi Auto Transformation) Vector System used the oncogenes of Agrobacterium to select transgenic plants. But there is a problem because the oncogenes cause abnormal phenotypes in the transgenic plants. Thus, the MAT vector with the site- specific recombination system R / RS to removed these genes (oncogenes) from transgenic plants after transformation and then produce of normal phenotype in transgenic plants. In this review article, we have evaluated the MAT vector system as a transformation method in plants to generate marker- free transgenic plants.

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Conclusion

In conventional transformation systems selected marker genes are essential for the introduce of genes with high economic value to plants and they are very important for the selection of transgenic plants. For example, antibiotics or herbicide genes are selectable markers that they are required to select non-transgenic and transgenic cells. but often these markers leads to negative effects in transgenic or non- transgenic cells. For example, these genes are destroyed non- transgenic cells or may also have negative effects on the development of transgenic cells. Until now, after transformation many selected marker genes remained in transgenic plants. So this selection methods have two major disadvantages: 1. It reduces the ability of plant cells to proliferation and differentiation 2. The uncertainty of the amount of their negative effects on the environment. Recently, number of gene transformation systems are used to produce marker-free transgenic plants. One of these systems is MAT vec

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How to cite this article

Zouhreh Poudine, Atefeh Galavi, Mehrdad Asadian and Hassan Shahgholi (2016). MAT vector: a system for production and selection of marker free transgenic plants- A survey and summary, Biological Forum – An International Journal ,8(1): 32-34.