In vitro Qualitative and Quantitative Assessment of PGPR Potential Attributes against Rhizoctonia bataticola in Chickpea

Author: Bethala Kumeera, Swapnil Matikhaye, P.W. Ramteke and Suchit A. John

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Abstract

The present study confirmed that out of thirty one isolates collected from chickpea rhizoshpere, four isolates PR7, PR10, PR30, PR31 found to be potent and identified PR7, PR10 as E. cloacae gram negative and PR30, PR31 as B. subtills gram positive in clear microscopic view at 100x magnification. These strains were identified on basis of 16s rRNA sequencing and were resulted to be antagonistic against R. bataicola with 65.75% of higher zone inhibition observed in PR30 strain. Protease ranged between 27.75 to 19.00 μg/ml of production, siderophore with 1.29 in PR30 to 0.98 μg/ml which helps in iron uptake, and low production in PR7. Chitin assay was 192.25 to 147.75 U/ml of production reported high in PR7, Cellulase was 482.0 U/ml observed in PR30 assisted in production of lytic enzymes that is responsible for breakdown of fungal cell wall. Whereas HCN was only able to change color in PR10 only. All these mentioned four isolates in this study, performed PGPR traits such as assessment

Keywords

PGPR, R. bataticola, E. cloacae, B. subtills, Chickpea, Qualitative and Quantitative traits.

Conclusion

Plant growth promoting rhizobacteria (PGPR) has a prominent role but dominated by harsh chemicals, pesticides to manage dry root rot caused by Rhizoctonia bataticola, which negatively impacts on environment and crop productivity. Due to vast usage of these agrochemicals, a serious threat facing in global and health issues annually. Inorder to overcome this criteria, PGPR is an alternative solution for managing soil borne fungal pathogen, thus being beneficial for crop growth, yield in chickpea as well as significantly helpful for marginal farmers in present agricultural scenario organically and even to reduce environment and health concerns. In this present study, out of thirty one root colonizing chickpea (C. arietinum) rhizosphere bacterial isolates, four isolates reported highly antagonistic activity by suppressing R. bataticola growth mainly by showing its bio-control activity under in vitro conditions. PGPR attributes including of both indirect and direct traits, all these four me

References

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How to cite this article

Kumeera, B., Matikhaye, S., Ramteke, P.W. and John, S.A. (2021). In vitro Qualitative and Quantitative Assessment of PGPR Potential Attributes against Rhizoctonia bataticola in Chickpea. Biological Forum – An International Journal, 13(3): 233-240.