Expression of CAT gene Resistance against Fusarium Wilt in Pigeonpea

Author: Prashant Gigaulia, Keerti Tantwai, Sushma Nema and Sanjay Kumar Singh

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Abstract

Wilt is most common and serious disease in pigeonpea. Many published reports have indicated 30-100% yield loss due to wilt in pigeonpea. Study of molecular markers and expression level of associated genes are very important to overcome the problem of wilt disease in pigeonpea. The causal organism of Fusarium wilt in pigeonpea is Fusarium udum. We report the efficacy of SSR markers among 14 pigeonpea varieties and expression analysis of Fusarium wilt resistance gene done by qRT-PCR. 14 varieties of pigeonpea were collected and analyzed using 13 SSR primers. Fusarium udum was isolated from infected plants of pigeonpea. Three varieties ICP-2376, Asha, and TJT-501 were grown in infected and control condition for expression analysis. Inoculation was done by root dipping into fungal spore suspension culture at four different time intervals viz. 0, 48, 72 and 120 hours. Total RNA was extracted from root parts. The expression level of CAT gene was tested in all the selected variety at four dif

Keywords

Pigeonpea, SSR Markers, wilt resistance gene, Fusarium udum, qRT-PCR.

Conclusion

Identification of different wilt resistant and wilt susceptible pigeonpea varieties was achieved by the use of SSR markers. SSR marker helps in the selection of diverse pigeonpea varieties for crop improvement programme and also useful for efficient screening of the germplasm. Total 18 different alleles were generated using 13 SSR primers. It is also concluded that Fusarium udum can easily and rapidly grow in Potato Dextrose Agar (PDA) media at optimum temperature and light. The inoculation procedure for causing wilt disease in plants by spore suspension method was also optimized. Wilt resistant gene present in wilt resistant plant expresses when fungal attack or biotic stress occurs. The expression of genes correlates with infection levels in response to changes in environmental conditions even in control condition. The qRT-PCR technique has validated its potential for further use in gene expression analysis of other targeted genes involved in pigeonpea against wilt disease. The polym

References

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How to cite this article

Gigaulia, P., Tantwai, K., Nema, S. and Singh, S.K. (2021). Expression of CAT gene Resistance against Fusarium Wilt in Pigeonpea. Biological Forum – An International Journal, 13(3): 255-261.