In vitro Evaluation of Bio-efficacy of Botanicals as Water and Ether extracts against Fusarium oxysporum f. sp. radicis-cucumerinum

Author: Suresh Kumar, N.L. Meena, Rohith M., Laxmi Choudhary, Akansha Deora and Kalpana Yadav

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Abstract

Use of natural products like botanical amendments or botanical extracts for the management of fungal diseases in plants is considered as a substitute method to synthetic fungicides, due to their less negative effects on the human and environment health hazard or implications. Botanicals are gaining importance in crop protection in view of their selective properties, low cost and safety to ecosystem. The antifungal properties of plants have been proved in a number of instances as potential means for the control of many diseases. The present study was conducted to evaluate the antifungal efficacy of few botanicals extracted using water and ether against Fusarium oxysporum f. sp. radicis-cucumerinum in vitro. To study the antifungal mechanism of plant extract, poisoned food technique was used for experiment. Eight plant species viz., Ipomea carnea, Calotropis gigantean, Allium cepa, Datura stromonium, Catharanthus roseus, Azadirachta indica, Curcuma longa and Piper nigrum used to test the

Keywords

botanicals, water and ether extract, Fusarium oxysporum f. sp. radicis-cucumerinum.

Conclusion

To develop effective management strategies, the botanicals should be included in the cultivation practice. Eight Botanicals as water and Ether extract such as Ipomea carnea, Calotropis gigantea, Allium cepa, Datura stromonium, Catharanthus roseus, Azadirachta indica, Curcuma longa and Piper nigrum were evaluated in vitro against mycelial growth of F. oxysporum f. sp. radicis-cucumerinum at three concentrations viz., 10, 20 and 30 per cent by poison food technique. Among them, A. indica ether extract was highly effective in inhibiting the growth of Pathogen as 62.96, 74.07 and 82.22 percent at 10, 20 and 30 percent concentrations, respectively. And in water extracts, A. indica extract also found effective in inhibiting the growth of pathogen as 58.51, 69.63 and 77.77 percent at 10, 20 and 30 percent concentrations, respectively. The other botanicals were not so effective in inhibiting the growth of the pathogen, the only other botanical that exhibited good per cent inhibition of the pat

References

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How to cite this article

Kumar, S., Meena, N.L., Rohith M., Choudhary, L., Deora, A. and Yadav, K. (2021). In vitro Evaluation of Bio-efficacy of Botanicals as Water and ether extracts against Fusarium oxysporum f. sp. radicis cucumerinum. Biological Forum – An International Jo