Author: Anamika Walia, Anil Bhushan, R.K. Samnotra, Anshika Gaur and Pankaj Pandotra
Production of quality tubers, free from diseases and pests in potato is one of the foremost pre-conditions for obtaining good crop yields in the field. However, field multiplied tubers have low multiplication rate and prevalence of diseases and virus has been recognized as one of the major bottlenecks in potato cultivation in developing countries. An alternative method of production i.e., micropropagation can be effectively utilized for mass production of true-to-type, disease free quality planting material of potato. For the experiments on micropropagation, two explants viz., sprouts and nodal segments were cultured on MS basal medium supplemented with different hormonal combinations (BAP and kinetin). The best shoot initiation response was recorded on MS medium supplemented with BAP 0.75 mg/l in both the explants. For shoot multiplication the best response was obtained on MS medium supplemented with 0.75 mg/l BAP + 0.25 mg/l kinetin. For rooting, superior percentage rooting response
Potato; Micropropagation; in vitro; sprouts; nodal segments; Kufri Girdhari.
A micropropagation protocol for potato variety Kufri Girdhari was optimized from the study. This protocol will provide the base for the disease free and high-quality mass production of studied variety through in vitro technique.
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Walia, A., Bhushan, A., Samnotra, R.K., Gaur, A. and Pandotra, P. (2021). In Vitro Propagation Studies for Late Blight Resistant Potato Variety Kufri Girdhari. Biological Forum – An International Journal, 13(3): 375-380.