Studies on Effect of Growing Environments and Storage Conditions on Assessment of Pollen Viability using Different Staining Techniques in Tomato

Author: Jorrigal Laxman, Shiv K. Yadav*, Ravish Choudhary, Sangita Yadav, Zakir Hussian, P.K. Singh, Sushil Pandey and Sunil Archak

Download PDF

Abstract

The main objective of tomato pollen storage is to preserve the genetic material for future use, to maintain their germination, vigour and genetic integrity under optimal conditions. Major challenge faced by tomato breeding program to develop new hybrids is time consuming and difficult hand emasculation and pollination for out-crossing occurs. This is because of the cleistogamous nature of flowers wherein, no cross-pollination occurs. Another challenge is of less pollen availability in tomato flowers during pollination. Multiple planting and staggered sowing can evade this constraint to some level but it is labour, time, and space intensive. Thus, accessibility of stored viable pollen for the breeding program can be of great help as it enables the easy exchange of genetic material between the researchers due to fewer stringent restrictions on the transportation. In the present study, viability of pollen produced in different growing environments and stored in variable conditions were a

Keywords

Cleistogamous, environmental conditions, iodine potassium iodide, pollen viability, room temperature, storage conditions, tetrazolium, tomato, PFS= percent fruit set

Conclusion

This is the first report on the tomato pollen collected from different environments followed by its storage at different storage conditions and evaluation of pollen viability. The results revealed that the pollen viability percentage is more in the polyhouse than the net house and open field conditions. Furthermore refrigerated (4°C) condition is more preferable storage strategy for effective preservation of nuclear genetic diversity and for pollination in the hybridization programme. Among the different staining techniques acetocarmine is more reliable and preferable than other two staining methods. These results will help the breeders to plan hybridization programme in tomato crop and further studies can be done on pollen viability using different staining techniques like FDA, in vitro germination across environments. Identifying heat stress tolerance male lines producing and maintaining viability of pollens for commercial hybrid seed production for open field conditions a

References

-

How to cite this article

Laxman, J., Yadav, S.K., Choudhary, R., Yadav, S., Hussian, Z., Singh, P.K., Pandey, S. and Archak, S. (2021). Studies on Effect of Growing Environments and Storage Conditions on Assessment of Pollen Viability using Different Staining Techniques in Tomato