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Evaluation of Different Culture Media on the Growth, Sporulation and Mycelial Dry Weight of Alternaria solani causing Early Blight in Tomato

Author: Ambarish K.V.*, Patil M.B., Amaresh Y.S., Pramesh D., Patil M.G. and Sreenivas, A.G.

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Abstract

Early blight caused by Alternaria solani is the most prevalent and destructive disease in tomato. Any study leading to an understanding of the host-pathogen connection must have a critical and complete grasp of dietary patterns and factors driving fungal growth. The pathogen's culture and growth media conditions have not received significant attention. Hence, 17 solid culture media (synthetic and non synthetic media) were tested to assess their influence on cultural characters of A. solani, and the results showed that mean colony diameter (9 cm) was significantly highest on Richard's agar medium, PDA and host based media at 12 DAI. The least growth of 1.72 cm was noticed in PDA+ CaCO3, followed by water agar (4.95 cm) and V8 juice agar (5.00 cm). A medium mycelial growth range of 6.45 to 8.80 cm was noticed in the rest of tested culture media. On examination of data on sporulation of A. solani, water agar showed excellent sporulation with 56.67 spores per microscopic field. Whereas poo

Keywords

Early blight, Alternaria solani, culture medium, Growth, sporulation and mycelial dry weight

Conclusion

In the current investigation, a total of 17 different culture media were tested to assess their influence on A. solani cultural characters and sporulation. Colony diameter (9 cm) was significantly highest on Richard's agar medium, PDA and host based media at 12 DAI. Similarly, water agar showed excellent sporulation with 56.67 spores per microscopic field and Sabourd's broth recorded maximum mycelium dry weight of 333.68 mg at 12 DAI.

References

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How to cite this article

Ambarish, K.V., Patil, M.B., Amaresh, Y.S., Pramesh, D., Patil, M.G., Sreenivas, A.G. (2021). Evaluation of Different Culture Media on the Growth, Sporulation and Mycelial Dry Weight of Alternaria solani causing Early Blight in Tomato. Biological Forum â€