Development of Yeast Based Biocontrol Formulation for Post Harvest Disease Management with Osmotic and Oxidative Stress Tolerance

Author: Jasmin Thomas*, Sanam Halsana, Deewakar Baral and Jayanta Saha

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Abstract

The yeast based biocontrol product is safe alternative to synthetic chemicals against post-harvest diseases. Antagonistic yeasts are exposed to a wide range of adverse stresses during pre and post-harvest application, as well as during the production process. Tolerance to environmental stresses is a prerequisite for successful development of biocontrol yeast formulation. An investigation was carried out to develop a stable liquid formulation of the antagonistic yeast isolate Sc YZ 7 (Saccharomyces cerevisiae) with high shelf life and osmotic and oxidative stress tolerance capacity. Osmotic stress tolerance of Sc YZ 7 yeast isolate was evaluated by changing the concentration of molasses and also with different concentrations of Sodium chloride in molasses urea medium. The number of viable cells (CFU ml-1) was determined using modified serial dilution method. The formulation prepared with yeast isolate had highest viability in 1% NaCl and 15% molasses added separately upto 120 days of st

Keywords

Antagonistic Yeast, Osmotic stress, Oxidative stress, Biocontrol formulation, ecofriendly management.

Conclusion

The yeast isolate Sc YZ7 (Saccharomyces cerevisiae) was found to be resilient to both osmotic and oxidative stress in the formulation in this study. As a result, it could be employed as a biocontrol stain in the creation of formulations. The adaptation to the stressed environment may be due to the accumulation of antistress compounds in the cells. The presence of additives like sorbitol, glycerol and ascorbic acid showed some protective effect against stresses. The molasses urea based medium is good for yeast cell mass multiplication and is inexpensive enough to be employed in commercial production. It is an environmentally friendly method of managing post-harvest fungal diseases. A detailed study on physiological changes and metabolism of yeast cells during osmotic and oxidative stresses will contribute the development of yeast based biocontrol formulation with longer shelf life as well as provide insight into antistress compounds.

References

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How to cite this article

Thomas, J., Halsana, S., Baral, D. and Saha, J. (2021). Development of Yeast Based Biocontrol Formulation for Post Harvest Disease Management with Osmotic and Oxidative Stress Tolerance. Biological Forum – An International Journal, 13(3a): 47-52.