Genetic Diversity Appraisal in Robusta Coffee (Coffea canephora var. S.274) Beans using Random Amplified Polymorphic DNA Markers

Author: S.B. Hareesh* and Jayarama

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Abstract

Coffee is a most extensively accepted beverage for many centuries. As an export-oriented commodity, coffee plays key position in the national economies of nearly 80 developing countries in the world involved in its production. Indian Coffees are renowned as the world’s best shade-grown ‘mild’ coffees. Several studies conducted earlier have signified that molecular biology approaches based on DNA detection methods play a dynamic role in varietal identification, food safety control, and authentication. Molecular markers have been widely useful over recent years to endow with a better perceptive of the genetic basis of important agronomic traits in plants. Breeding programs in coffee are more concerned with the identification of agronomic traits such as quality, resistance to major pests, and diseases besides productivity. In coffee biotechnology, Random amplified polymorphic DNA (RAPDs) are useful in cultivar identification, population & pedigree analyses, phylogenetic studies and

Keywords

Robusta Coffee, Coffea canephora, RAPD, Genetic Diversity, Organic and Integrated nutrition management (INM) practices.

Conclusion

In the present study, DNA extraction was done from the green coffee of Robusta coffee (Coffea canephora var. S.274) beans. Further by DNA samples were subjected to Polymerase Chain Reaction (PCR) and amplification was done using random primers (RAPD) analysis. Twenty-five decamer primers were used to understand the genetic relatedness and differences among samples that are subjected to different treatments. These twelve primers gave consistent and clear amplification products, of which 70.4 percent bands were polymorphic and the remaining 24 percent bands were found to be monomorphic. On average each primer amplified an average of 6.75 bands, of which 4.75 bands were polymorphic. The RAPD amplification has revealed that Bot 18, Bot 22, and Bot 24 amplified four rare alleles in the samples analyzed. Hence only one variety of Robusta coffee (S.274) is chosen for the present study, RAPD pattern is also revealed relatively low genetic diversity in the Robusta coffee (Coffea canephora var.

References

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How to cite this article

Hareesh, S.B. and Jayarama (2021). Genetic Diversity Appraisal in Robusta Coffee (Coffea canephora var. S.274) Beans using Random Amplified Polymorphic DNA Markers. Biological Forum – An International Journal, 13(3a): 66-72.