Development of Sterilization and Initiation Protocol for in vitro Regeneration in Bamboo (Bambusa balcooa Roxb.)

Author: Ananthu J.S.*, S.V. Pawar, B.R. Najan, Y.M. Raut and Vaibhav Parmeshwar Gulwane

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Abstract

In bamboo, propagation is usually done by two main methods. First by propagation of bamboos by using offsets, culm or side branch cuttings and second way is through micropropagation, also called as clonal propagation, which is best achieved through tissue culture techniques. In vitro propagation is frequently used for meeting the ever-growing demand for this bamboo species due to its multipurpose nature. The present investigation on development of sterilization and initiation protocol for in vitro regeneration in bamboo (Bambusa balcooa Roxb.) was undertaken to identify suitable explants and media for its micropropagation. The experiment was conducted using nodal explants collected from Bamboo Plantation Project, MPKV, Rahuri and the current research was conducted in State Level Biotechnology Centre, MPKV, Rahuri. Nodal explants as explants and MS media as basal media were found ideal for in vitro culture. In case of sterilisation of nodal explants, sterilisation done with Tween 20 (10

Keywords

Nodal explants, Sterilization, Initiation, MS media, Standard error.

Conclusion

Among different bamboos, Bambusa balcooa is one among the most important and desired species of farmer for raising plantation mainly due to its multipurpose utility. Since unknown past, the species has been extensively cultivated and became a very popular bamboo for the farmers. It grows well in tropical humid conditions. It also has a high Calorific value and Fuel Value Indices (FVI) which makes it suitable for Bio- energy plantations and gasification. Bambusa balcooa is suitable for paper and pulp industry. It shows superior strength properties including good culm wall thickness. Life cycle of clump is more than 50 years and also has an added advantage as it shows minimal pest problems and no gregarious flowering in India. Due to the increasing demand of Bambusa balcooa, in vitro regeneration of this bamboo has gained importance as it enables mass multiplication in a small period of time and thus it offsets the limitations of traditional propagation methods followed earlier. An ef

References

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How to cite this article

Ananthu J.S., Pawar, S.V., Najan, B.R., Raut, Y.M. and Gulwane, V.P. (2021). Development of Sterilization and Initiation Protocol for in vitro Regeneration in Bamboo (Bambusa balcooa Roxb.). Biological Forum – An International Journal, 13(3a): 221-227.