Response of Growth Hormones on Staghorn fern (Platycerium bifurcatum (Cav.) C.Chr.) Gametophytes under in vitro Condition

Author: Shiva Sai Prasad*, Gopinath G., Sathyanarayana, B.N., Devakumar, A.S. and Veena, S.A.

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Abstract

Staghorn fern is an ornamental fern which is epiphytic in nature and reproduce through transformation of generation, which happens over a long time. The present study aims to reduce the transformation phase of gametophyte to sporophyte by the influence of growth hormones under in vitro condition. The gametophytes of staghorn fern (Platycerium bifurcatum (Cav.) C. Chr.) were subjected to three different growth hormones such as auxin, gibberlin and cytokinin at different concentrations to enhance the transformation in short time. The results revealed that, the treatment with auxin at 1.0 mg l–1 BAP resulted in the higher transformation of gametophytes to sporophyte, but the cytokine in treated were further proliferated, without any transformation. However, gibberlins at 3.0 mg l-1 was found to be better with respect to broader leaves with taller sporophytes.

Keywords

Platycerium bifurcatum (Cav.) C.Chr., Staghorn fern, gametophyte, sporophyte, auxin, gibberlin, cytokinin.

Conclusion

From the study it has been found that Staghorn fern needs stimuli for the alternation of generation. In the natural process, it was found to be taking much time for transformation from one stage to another, while by external application of different growth regulators have led to a result in reducing the transformation time and better development of sporophytes. It is clearly evident that the gametophytes do respond to growth hormones. And there is a greater scope in future for developing protocol for sporophytes development in shorter time and hardening of plantlets, which is an important need of the hour.

References

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How to cite this article

Prasad, S.S., Gopinath G., Sathyanarayana, B.N., Devakumar, A.S. and Veena, S.A. (2021). Response of Growth Hormones on Staghorn fern (Platycerium bifurcatum (Cav.) C.Chr.) Gametophytes under in vitro Condition. Biological Forum – An International Journ