Effect of Explant and Media Factors on Direct Shoot Organogenesis in Garlic (Allium sativum L.) cv. Ooty 2

Author: Meena, S., Gnanam, R., Kavitha, M., Radhamani, T., Karthikeyan, M. and Rajesh, S.

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Abstract

Garlic (Allium sativum L.) is one of the oldest cultivated crops known for its medicinal values and important spice used in culinary. In garlic, cloves are widely used as seed material for planting which poses challenges in increasing the input cost of cultivation and also there is questionable availability of quality planting material for the farmers. The present study was conducted to optimize the choice of explant for the direct organogenesis in the garlic cultivar Ooty 2. Direct organogenesis in garlic promotes micropropagation for virus-free bulblet production which could be employed as seed material. Shoot tip and basal meristematic region explants from garlic cloves were studied for their in vitro responses to shoot organogenesis. Basal region explants produced the maximum number of shoots in different shoot induction media compared to shoot tips. Mean number of shoots produced per clove is 3.2 when cultured in MS media supplemented with 0.1 mg L-1 NAA and 1 mg L-1 BAP. This is

Keywords

Garlic (Allium sativum L.), shoot tip, basal meristematic region, direct organogenesis

Conclusion

Explant and media optimization for the in vitro direct shoot regeneration in garlic cultivar Ooty 2 was developed in this study. The explants showed differential responses for the shoot regeneration. The basal region explant of the garlic was found to be the efficient explant than the shoot tips. This could also be excised more easily than the shoot tips and adopted when exploited on large scale. This report on micropropagation study for direct shoot organogenesis support and advance further researches on garlic, as a source of secondary explants for in vitro bulblet induction and genetic transformation procedures. Since bulblet induction demands quality shoots on larger scale, these findings from the present study in a popular commercial cultivar like Ooty2 has significance for future research on microbulblets production that can serve as quality planting material for the farmers.

References

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How to cite this article

Meena, S., Gnanam, R., Kavitha, M., Radhamani, T., Karthikeyan, M. and Rajesh, S. (2021). Effect of Explant and Media Factors on Direct Shoot Organogenesis in Garlic (Allium sativum l.) cv. Ooty 2. Biological Forum – An International Journal, 13(3a): 74