Morphological and Molecular Evaluation of Medium Slender (MS) Rice Genotypes for Leaf Blast Disease Resistance

Author: Vishal Reddy, K. Mahantashivayogayya*, D. Pramesh, J.R. Diwan and B.V. Tembhurne

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Abstract

Over half of the world's population consumes rice as a staple food. Magnaporthe oryzae causes rice blast, which is one among the foremost destructive disease causing enormous yield losses to rice in several rice growing regions. Blast resistance is highly unreliable, with resistance frequently failing or weakening in field conditions, prompting a constant search for resistant donors/lines. The current research was carried out at Agricultural Research Station (ARS) Gangavati, to identify resistance among 22 medium slender rice genotypes alongside susceptible check HR-12 in uniform blast nursery (UBN). The disease reactions were recorded one week after inoculation, with Standard Evaluation Scale (SES) for leaf blast ranging 0-9, when the susceptible check (HR 12) was completely killed. None of the MS varieties shown resistant but three MS varieties viz. IET-26241, IET-25520 and Rp Bio-226 were shown moderately resistant reaction against blast. The five major blast resistant genes genetic

Keywords

Rice, Blast, Magnaporthe oryzae, MS, Genetic Frequency.

Conclusion

In the current investigation a total of 22 varieties were tested along with susceptible check (HR 12) by artificial inoculation in uniform blast nursery (UBN) for blast disease resistance, results revealed that IET-26241, IET-25520 and Rp-Bio 226 were found to be moderately resistant and molecular identification of blast resistant genes in promising genotypes divulge that out of 5 primers, Rp-Bio 226 gave positive bands with all the primers. GNV 10-89, IET-25520 and IET-26241 gave positive bands for Pi2-i, Pi9-i, RM 72 and RM 212 primers. IET-27438 gave positive bands for Zt56591 and Pi2-i primers. GGV-05-01 gave positive bands for Pi2-i and Pi9-i. and BPT-5204 gave positive bands for RM212 primer.

References

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How to cite this article

Reddy V., Mahantashivayogayya, K., Pramesh , D., Diwan, J.R. and Tembhurne , B.V. (2021). Morphological and Molecular Evaluation of Medium Slender (MS) Rice Genotypes for Leaf Blast Disease Resistance. Biological Forum – An International Journal, 13(3a