Comparative Evaluation of Different Cell lines for Adaptation of Orf virus vaccine strain Passaged in Primary lamb testes cells

Author: Golmei Poulinlu, Gnanavel Venkatesan*, Amit Kumar, Anand Kushwaha and D. Muthuchelvan

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Abstract

Orf virus (ORFV) is a poxvirus that causes non-systemic epitheliotropic disease in sheep and goats, resulting in economic losses to the welfare of farmed small ruminants and belongs to the genus Parapox virus and family Poxviridae. Vaccination is the only way to control orf infection in sheep and goats. In India, available vaccine for orf is produced from primary lamb testes cells (LT), which is not viable for scale up in large production and also virus yield varies from different batches. To overcome this, an attempt was undertaken to adapt and characterize ORFV vaccine strain in three different commonly available cell lines. In this study, LT adapted ORFV Mukteswar-59/05 vaccine strain was used for adaptation in Vero, Madin Darby Bovine Kidney (MDBK) and Baby Hamster Kidney (BHK-21) cell lines. In order to adapt vaccine virus in cell lines, it was blindly passaged up to 7 times and the presence of the virus and its replication was demonstrated by presence or appearance of characteris

Keywords

Primary lamb testes cells, Orf virus, BHK-21, MDBK, Vero, Cytopathic effects, Adaptation

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