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Genotype-media Interactions in Aromatic Rice (Oryza sativa L.) Landraces of India, Relation with hypoxic Germination

Author: Subhashree Das*, Banshidhar Pradhan, Simanchal Sahu, Kailash Chandra Samal, Selukash Parida and Sanghamitra Samantaray

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Abstract

An experiment to optimize media for callus induction frequency (CIF) and shoot regeneration efficiency (SRE) of ten aromatic rice genotypes including nine local landraces and one released variety of Odisha using mature seed embryos as explants in five strengths of 2,4-dichlorophenoxy acetic acid (2,4-D) ( 1.0mgl-1, 1.5mgl-1, 2.0mgl-1, 2.5mgl-1, 3.0mgl-1) and two concentrations of 6- benzylaminopurine (BAP) (1.0 mg-1 and 2.0 mgl-1) in combination with α- naphthalene acetic acid (NAA) and kinetin (Kn) in Murashige & Skoog (MS) medium was performed. MS medium with 2mgl-1of 2,4-D, 0.5mgl-1 of NAA, 0.1mgl-1 of Kn, 0.25mgl-1of BAP and half strength MS medium with 2mgl-1 BAP, 0.25 mgl-1 Kn, 0.1mgl-1 NAA was found most efficient for CIF and SRE respectively. Tested genotypes showed varied responses towards CIF, SRE and callus morphology in the same medium. The response of genotypes to callus induction and shoot regeneration was supported by their behavior towards hypoxic germinati

Keywords

Aromatic rice, callus induction, regeneration, MS medium, hypoxic.

Conclusion

Both CIF and SRE are genotype dependent. Genes responsible for callus induction are different from shoot regeneration. Each genotype has its specific media requirement for efficient CIF and SRE. The positive association of anaerobic (hypoxic) germination capacity with CIF and seedling development with SRE of the genotypes may be due to the presence of QTLs associated with these characters. This relationship can be helpful while selecting a genotype for research program involving in vitro techniques.

References

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How to cite this article

Das, S., Pradhan, B., Sahu, S., Samal, K.C., Parida, S., and Samantaray, S. (2021). Genotype-media Interactions in Aromatic Rice (Oryza sativa L.) Landraces of India, Relation with hypoxic Germination. Biological Forum – An International Journal, 13(3a)