QTLs Analysis Controlling Physiological Traits of Barley under Arsenic Stress

Author: Fahime Mousavi*, Brat Ali Fakheri* and Farshid Golshani*

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Abstract

Arsenic (As) is a metalloid substance that can exist in inorganic or organic form and results in heavy-metal-induced plant toxicity via reactive oxygen species production. A population of 72 F1 double haploids derived from the Morex × Steptoe cross, and the two parental lines ,was used to identify the quantitative trait loci (QTLs) associated with arsenic tolerance, and determine the contribution of each QTL on trait diversity in the barley. This study were carried outunder normal and arsenic stress conditions, in two randomized complete block designs with three replications under hydroponic system at Agricultural Biotechnology Research Institute, Zabol University, Zabol, Iran, in 2013. physiological traits including Chlorophyll content, proline content, water soluble carbohydrate (WSC), relative water content (RWC), cell membrane stability (CMS) and chlorophyll fluorescence (Fo, Fm) were measured. QTL analysis was performed using the genetic linkage map derived from molecular marker

Keywords

Arsenic stress, Barley, Physiological traits, QTL mapping

Conclusion

The present study was the first investigation on QTL analysis of physiological traits of double haploid population of barley under the arsenic stress condition in Iran. In total, 23 QTLs were detected for studied traits. QTLs QFo5H.n and QFo5H.s is ranged from 148.8 cM of chromosome 5H, controlling Fo and QTLs Qch7H.n and Qch7H.s in Location from 14.01 cM of chromosome 7H, controlling Chlorophyll contents were quite stable. Therefore, if this result would repeat in different environment, years and genotypes, it can be used in marker assisted selection.

References

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How to cite this article

Fahime Mousavi, Brat Ali Fakheri* and Farshid Golshani (2016). QTLs Analysis Controlling Physiological Traits of Barley under Arsenic Stress , Biological Forum – An International Journal 8(1): 273-282.