Screening fluorescent pseudomonads isolated from wheat rhizosphere for plant growth- promoting and salt tolerance properties

Author: Dariush Safari*, Fatemeh Jamali**, Hamid-Reza Nooryazdan*** and Fereshteh Bayat***

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Abstract

30 fluorescent pseudomonads were isolated from wheat rhizosphere in Bushehr province, Iran. Strains were screened for the key features of plant growth promotion including production of hydrogen cyanide and siderophore and inorganic phosphate solubilization under in vitro conditions. Then, superior strains were evaluated for production of indole acetic acid (IAA). Results revealed that 100% of isolates were able to produce siderophore, 74.44% produced hydrogen cyanide and 96.66% were capable of solubilizing inorganic phosphate. All superior PGPR strains evaluated for IAA production were able to produce the metabolite. 30 Pseudomonas strains isolated in this study, were evaluated for tolerance to salinity levels of 200, 400 and 600 mM sodium chloride (EC equivalent, respectively, 9/28, 3/47 and 2/61 ds/m). Results showed that all strains could tolerate 400 mM NaCl concentrations and had a weak growth at 600 mM (classified as semi-tolerant), but none of them could grow strongly on 600 mM

Keywords

Wheat, salt stress, siderophore, hydrogen cyanide, fluorescent pseudomonads

Conclusion

30 fluorescent pseudomonads were isolated from wheat rhizosphere in Bushehr province, Iran. Strains were screened for the key features of plant growth promotion including production of hydrogen cyanide and siderophore and inorganic phosphate solubilization under in vitro conditions. Then, superior strains were evaluated for production of indole acetic acid (IAA). Results revealed that 100% of isolates were able to produce siderophore, 74.44% produced hydrogen cyanide and 96.66% were capable of solubilizing inorganic phosphate. All superior PGPR strains evaluated for IAA production were able to produce the metabolite. 30 Pseudomonas strains isolated in this study, were evaluated for tolerance to salinity levels of 200, 400 and 600 mM sodium chloride (EC equivalent, respectively, 9/28, 3/47 and 2/61 ds/m). Results showed that all strains could tolerate 400 mM NaCl concentrations and had a weak growth at 600 mM (classified as semi-tolerant), but none of them could grow strongly on 600 mM

References

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How to cite this article

Dariush Safari, Fatemeh Jamali, Hamid-Reza Nooryazdan and Fereshteh Bayat (2016) Screening fluorescent pseudomonads isolated from wheat rhizosphere for plant growth- promoting and salt tolerance properties Biological Forum – An International Journal 8(1