Seasonal Studies on Antimicrobial Activities of Crude Hexane and Ethanolic Leaf Extracts of Acacia nilotica L. against E. coli, Staphylococcus aureus and Candida albicans

Author: Deepika Rana, P.K. Chauhan and Mohamed Azhar Khan

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Abstract

Acacia nilotica has been used by locals over the years to treat several microbial infections. The fresh plant parts of Acacia nilotica is considered as astringent, demulcent, aphrodisiac, anthelmintic, antimicrobial, antidiarrhoeal, with good nutritional value in Indian traditional medicine system. In the present study antimicrobial activity of the crude extracts of six samples of Acacia nilotica were screened against two bacterial strains, E. coli (gram –ve), Staphylococcus aureus (gram +ve) and one fungal strain, Candida albicans. The leaves were collected in different seasons from six regions of Himachal Pradesh. Antimicrobial activity was determined by well diffusion method. The concentration of plant extract (75mg/ml) was mixed with DMSO and added into the well. The inhibitory effect of ethanolic extract of Acacia nilotica was relatively higher than that of hexane extract. Combined effect of plant extract and drug was also determined. Leaves extract exhibited considerable bacter

Keywords

Herbal extracts, fungicide, Acacia nilotica, zone of inhibition.

Conclusion

The present investigation on antimicrobial activity of herbal plant extracts of Acacia nilotica showed that ethanol extract showed promising antibacterial activity against E. coli and S. aureus in comparison to hexane extract. Ethanolic leaf extract of A. nilotica at 75mg/ml concentration was found effective for E. coli and S. aureus. However, this concentration was found ineffective for C. albicans. This study justifies the traditional use of Acacia nilotica in medicine for treatment various infectious diseases caused by the microbes.

References

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How to cite this article

Deepika Rana, P.K. Chauhan and Mohamed Azhar Khan (2016). Seasonal Studies on Antimicrobial Activities of Crude Hexane and Ethanolic Leaf Extracts of Acacia nilotica L. against E. coli, Staphylococcus aureus and Candida albicans , Biological Forum – A