Studies on Salicylic Acid as a Possible Biochemical Marker for Screening Tobacco Mosaic Virus Resistant Progenies in Tobacco Breeding

Author: K. N. Subrahmanya* and M. Krishnappa**

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Abstract

Salicylic acid (SA) has gained importance from the discoveries as an endogenous regulator of flowering in thermogenic plants. Induction of resistance to Tobacco Mosaic Virus in tobacco by exogenous application of SA and demonstration of its accumulation at the sight of necrosis following infection in tobacco plants have given a new dimension to its role in resistance. In the present study, a comparative estimation of SA in TMV resistant, tolerant and susceptible varieties was made to explore the feasibility of its use as a marker to distinguish differential response of genotypes to TMV infection. There was no difference in the basal level of SA in leaves, among varieties. However, only resistant but neither susceptible nor tolerant variety showed significant accumulation of SA after TMV inoculation and increase was 117% above the levels found in leaves of healthy plants i.e., from 0.43 to 0.94 µg/g fresh weight. To validate the practical utility of this biochemical marker in breeding

Keywords

Salicylic Acid, Marker, Tobacco Mosaic Virus, Resistance, Tolerance, Tobacco Breeding

Conclusion

Salicylic acid (SA) has gained importance from the discoveries as an endogenous regulator of flowering in thermogenic plants. Induction of resistance to Tobacco Mosaic Virus in tobacco by exogenous application of SA and demonstration of its accumulation at the sight of necrosis following infection in tobacco plants have given a new dimension to its role in resistance. In the present study, a comparative estimation of SA in TMV resistant, tolerant and susceptible varieties was made to explore the feasibility of its use as a marker to distinguish differential response of genotypes to TMV infection. There was no difference in the basal level of SA in leaves, among varieties. However, only resistant but neither susceptible nor tolerant variety showed significant accumulation of SA after TMV inoculation and increase was 117% above the levels found in leaves of healthy plants i.e., from 0.43 to 0.94 µg/g fresh weight. To validate the practical utility of this biochemical marker in breeding

References

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How to cite this article

K. N. Subrahmanya and M. Krishnappa (2016). Studies on Salicylic Acid as a Possible Biochemical Marker for Screening Tobacco Mosaic Virus Resistant Progenies in Tobacco Breeding , Biological Forum – An International Journal 8(1): 386-390.