Studies on the Effect of different Media on the Mycelial Growth of Colletotrichum lindemuthianum

Author: Sonali Bhagat, S.K. Singh, Sneha Choudhary, Ranbir Singh, V.B. Singh, A.K. Singh and Amrish Vaid

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Abstract

French bean anthracnose is one of the major disease caused by Colletotrichum lindemuthianum and the pathogen is responsible for causing significant yield losses. C. lindemuthianum is a slow growing pathogen and is usually grown on Potato Dextrose Agar medium, thus in vitro studies were conducted to study the mycelial growth and colony characters of the fungus on six different media viz., Potato Dextrose Agar, Czapek’s Dox Agar, Oat Meal Agar, Richard’s Synthetic Agar, Fungal Agar and Potato Carrot Agar. The result revealed that the mycelial growth in all the media ranged between 62.33 mm to 90.00 mm. Among all the tested media, Potato Dextrose Agar recorded the maximum mycelial growth of 90.00 mm. However, Potato Carrot Agar recorded the minimum mycelial growth of 62.33 mm. The colony characters viz., growth pattern, colony margin and colony colour was also recorded. The fungus produced cottony to fluffy growth, regular to irregular margin and the colony colour varied from white to whitish gray. Potato Dextrose Agar proved to be the best suitable media for the growth of C. lindemuthianum.

Keywords

French bean, anthracnose, Colletotrichum lindemuthianum, media

Conclusion

The study revealed that among all the tested media, Potato Dextrose Agar showed the best suitability for the growth of C. lindemuthianum and recorded the maximum mycelial growth after 15 days of incubation.

References

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How to cite this article

Sonali Bhagat, S.K. Singh, Sneha Choudhary, Ranbir Singh, V.B. Singh, A.K. Singh and Amrish Vaid (2022). Studies on the Effect of different Media on the Mycelial Growth of Colletotrichum lindemuthianum. Biological Forum – An International Journal, 14(4): 143-145.