Efficiency of Pesticide Degrading Probiotic Lactobacillus plantarum Pb3 to Remove Chlorpyriphos on Tomato Fruits

Author: Palanisamy M.* and Vijila K.

Download PDF

Abstract

Food contamination is a global major concern in food health and safety. Therefore, the present study was aimed to evaluate the potential ability of probiotics in pesticides detoxification. In this context we investigated the reduction of chlorpyriphos residues on tomato fruits inoculated with Lactic acid bacteria (LAB). Lactobacillus plantarum Pb3 isolated from bhendi that received high amount of pesticide residues was used in this study. The strain, L. plantarum Pb3 possessed good tolerance to pesticides and the highest chlorpyriphos removal capacity. The L. plantarum Pb3 strain can bind chlorpyriphos as indicated by Gas chromatography analysis. The sample were prepared by QuEChERS method for extraction and clean-up process. The strain was employed for further in vivo studies on chlorpyriphos binding characteristics on tomato fruits. Water washing removed approximately 34.6% of pesticides added at a final concentration of 1 mg L-1 of tomato fruits. However, 67.8% of exogenously added chlorpyriphos was removed within 4h by heat-treated cells of L. plantarum Pb3. Heat treatment enhanced chlorpyriphos binding rate. During the biosorption process, tomato fruits did not exhibit any obvious adverse effects. These findings indicated that L. plantarum Pb3 may be useful to reduce chlorpyriphos in contaminated agricultural products.

Keywords

Lactic acid bacteria, Pesticide, chlorpyriphos, binding, Tomato

Conclusion

In summary, the chlorpyripos binding capacity of the probiotic strain of L. plantarum Pb3 on tomato was determined in this study. The results showed that L. plantarum Pb3 manifested relatively excellent binding capacity to chlorpyriphos. The binding capacity would have been influenced by some factors, for instance, heat treatment enhanced the binding capacity. The binding process was reversible, and the bound chlorpyriphos could be washed. This study indicated that this strain is promising for reducing chlorpyriphos contamination on other food products also and decreasing the bioavailability of chlorpyriphos in food. In this approach cost-effective, ecological, and non-destructive when compared to physical and chemical remediation methods.

References

-

How to cite this article

Palanisamy M. and Vijila K. (2022). Efficiency of Pesticide Degrading Probiotic Lactobacillus plantarum Pb3 to Remove Chlorpyriphos on Tomato Fruits. Biological Forum – An International Journal, 14(4): 177-181.