Response Surface Methodological Study of Glucose Laurate Synthesis Catalyzed by Immobilized Lipase from Candida cylindracea

Author: Nadia Bouzaouit and Chahra Bidjou-Haiour

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Abstract

The purpose of the present work is to study the reaction conditions affecting the lipase-catalyzed synthesis of fatty acid glucose ester with immobilized Candida cylindracea lipase, using response surface methodology. According to the Box-Behnken design principles, response surface methodology with three factors such as temperature, solvent polarity and reaction time at three levels was adopted. The factors influencing the parameters were determined by means of regression analysis. The optimum values for the highest conversion yield of C=76% was obtained at a temperature of 80°C in ethyl methyl ketone as a solvent and at a reaction time of 72h. It was shown that the obtained model can predict conversion of the starting material and the obtained results were in good agreement to measured data.

Keywords

Lipase from Candida cylindracea, sugar, optimization, response surface methodology.

Conclusion

The modeling and optimization of immobilized Candida cylindracea lipase catalyzed synthesis of glucose ester was successfully performed using a response surface methodology based on a Box-Behnken design. Predictability of the results for the above-mentioned reaction was found to be very good. Under the optimal conditions, the conversion was 76% in EMK at 80°C and 72h. The determination coefficient (R2) was 0.923, which ensure an adequate credibility of the model. The effectiveness of the bio-catalytic esterification of D-Glucose with immobilized CCL was found to depend strongly on the reaction temperature and the solubility of the substrate.

References

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How to cite this article

Nadia Bouzaouit and Chahra Bidjou-Haiour (2016). Response Surface Methodological Study of Glucose Laurate Synthesis Catalyzed by Immobilized Lipase from Candida cylindracea Biological Forum – An International Journal 8(1): 420-427.