Management of Cercospora Leaf Spot in Mungbean by use of Botanicals, Fungicides and Bio-agents

Author: Rahul Singh Raghuvanshi, Subhash Chandra, Abhishek Singh, Vivek Singh, Sakshi Pandey and Vishwa Vijay Raghuvanshi

Download PDF

Abstract

This study aimed to investigate the efficiency of Management of Cercospora leaf spot in mungbean by use of botanicals, fungicides and bio-agents. The mungbean crop is infected by a large number of pathogens such as fungi, bacteria, virus and nematodes in which mungbean Cercospora Leaf Spot causes significantly losses. At present Cercospora Leaf Spot is being managed by using fungicide through seed and soil treatment. However, fungicides are more costly and pollutant to environment. Many plant extracts are known to have antifungal activity. During the present investigation, extract of 5 plants species namely, Neem, Garlic, Tulsi, Onion, Ginger were evaluated for fungi toxicity against C. canescens by using poison food technique. The per cent inhibition in radial growth of Cercospora canescens was maximum in Garlic (85.22 %), followed by Tulsi (84.35 %), Onion (84.13 %), Ginger (83.48 %), Neem (72.61 %). The minimum growth is recorded in Neem (58.51), however Ginger and Onion, Tulsi, Onion and Tulsi, Garlic, Tulsi and Garlic were significantly at par to each other, Garlic was found significantly superior at 10 per cent concentration after 7 days of incubation. The efficacy of chemicals in vitro revealed that Carbendazim completely inhibited the mycelial growth of C. canescens at 200 ppm concentration. Bio-agents, Trichoderma viride and Trichoderma harzianum were evaluated by using dual culture technique against Cercospora canescens causing leaf spot of mungbean. The radial growth were (10.20 mm) and (19.30 mm) in T. viride and T. harzianum, respectively at 7 days of incubation which differed significantly from each other.

Keywords

Botanicals, Garlic, Cercospora, Bio-agent, Chemicals

Conclusion

1. The efficacy of botanicals Five plant extracts viz., Neem, Garlic, Tulsi, Onion, Ginger were tested in vitro against Cercospora canescens. Food poison technique. All the five plant extracts showed significant reduction in radial growth of test fungus at different concentrations (5.00 and 10.00 %). The affectivity of extracts increased with an increase in concentration and time of incubation (4 and 7 days). (a) At 10.00 per cent concentration minimum radial growth was obtained in Garlic (5.10 mm) followed by Tulsi (5.40 mm), Onion (5.50 mm), Ginger (5.70 mm), Neem (9.50 mm), compared to control ( 42.00 mm), Each treatment significantly superior to control. Garlic and Tulsi, Onion, Tulsi and Onion, Ginger, Onion and Ginger were significantly at par to each other, however Ginger and Neem statistically different to each other. (b) The per cent inhibition in radial growth of C. canescens was maximum in Garlic (85.22 %), followed by Tulsi (84.35 %), Onion (84.13 %), Ginger (83.48 %), Neem (72.61 %). The minimum growth is recorded in Neem (58.51), however Ginger and Onion, Tulsi, Onion and Tulsi, Garlic, Tulsi and Garlic were significantly at par to each other, Garlic was found significantly superior at 10 per cent concentration after 7 days of incubation. The similar trends were also observed in case of 5 per cent concentration after 4 days of incubation. 2. The efficacy of chemicals In vitro During assessment of two fungicides namely, Carbendazim and Thiram and against C. canescens revealed that Carbendazim completely inhibited the mycelial growth of C. canescens at 200 ppm concentration. while the 500 ppm concentration radial growth were completely inhibited, however Thiram provided the some growth at 500 ppm concentration. 3. The efficacy of bio-agents Trichoderma viride and T. harzianum were tested In vitro for growth and per cent inhibition of C. canescens. By usingdual culture technique. T. viridewas more effective as compared to T. harzianum in inhibiting the mycelial growth of C. canescens. 4 and 7days of incubation. (a) The radial growth was minimum in T. viride (7.50 mm) following by T. harzianum (10.20mm) as compared to control (19.30 mm) which were statistically different from each other at 7 days of incubation (b) Trichoderma viride showed (75.71 %) per cent inhibition as compared to T. harzianum (54.05 %) after 7 days of incubation which significantly differed to each other Similar results were also obtained after 4 days of incubation with respect to radial growth and per cent inhibition.

References

-

How to cite this article

Rahul Singh Raghuvanshi, Subhash Chandra, Abhishek Singh, Vivek Singh, Sakshi Pandey and Vishwa Vijay Raghuvanshi (2022). Management of Cercospora Leaf Spot in Mungbean by use of Botanicals, Fungicides and Bio-agents. Biological Forum – An International Journal, 14(4): 276-287.