In Vitro Efficacy of Biocontrol agents and Fungicides against the Brown Leaf Spot (Bipolaris oryzae) of Rice

Author: Sonali Abrol, S.K. Singh, V.B. Singh, A.K. Singh and Ranbir Singh

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Abstract

In tropical and sub-tropical countries rice production is considered as backbone of agricultural economy, including India, the world’s 2nd largest producer and consumer. In Indian sub-continent including Jammu and Kashmir region, rice is attacked by numerous plant pathogens. Among these, Bipolaris oryzae is responsible for causing brown leaf spot as one of the major and serious disease that causes significant quantitative and qualitative losses in rice. The present study was carried out to evaluate the biocontrol agents and different fungicides under in vitro conditions for their efficacy against brown leaf spot pathogen of rice (Bipolaris oryzae). The results showed that among the combo fungicides, highest growth inhibition was recorded in azoxystrobin + difenoconazole followed by propiconazole + difenoconazole and tebuconazole + trifloxystrobin at 250, 100, 50, 25 and 10ppm respectively. Among the single fungicides, propiconazole was most effective fungicide followed by azoxystrobin in inhibiting the mycelial growth of pathogen at 250, 100, 50, 25 and 10ppm, respectively. Further, among the fungal biocontrol agents maximum growth inhibition of pathogen was recorded in T. harzianum (Th-III) followed by T. harzianum (Th-II) and T. harzianum (Th-I). However, the inhibition efficiency was less when compared to fungicides.

Keywords

Biocontrol agents, Bipolaris oryzae, brown leaf spot, in vitro, T. harzianum

Conclusion

The present study was carried out to evaluate the effect of biocontrol agents and different fungicides against the mycelial growth inhibition of B. oryzae under in vitro conditions. All the biocontrol agents were effective in restricting the mycelial growth of pathogen compared to control. In vitro evaluation of different fungicide also revealed that all the fungicides inhibited mycelial growth of B. oryzae even at their lowest concentration. It was observed that with the increase in the concentration of fungicides, there was significant decrease in the respective mycelial growth and accordingly more inhibition was observed at higher concentrations.

References

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How to cite this article

Sonali Abrol, S.K. Singh, V.B. Singh, A.K. Singh and Ranbir Singh (2022). In Vitro Efficacy of Biocontrol Agents and Fungicides against the Brown Leaf Spot (Bipolaris oryzae) of Rice. Biological Forum – An International Journal, 14(4): 373-377.