Preparation of Low Cost Silicon Cast of Tracheo-Bronchial Tree of Barbari Goat

Author: Nimgaonkar Mayur Sudhirrao, K.N. Singh, Mukesh Kumar, R.K.Joshi, Pankaj Kumar Choudhary, Sonu Jaiswal and Amit Singh Vishen

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Abstract

The silicon cast of tracheo-bronchial tree is extremely imperative to understand the distribution of bronchial tree pattern. It may be an ideal model for comparative anatomical study of various species by silicon cast technique. Many different silicone and various methods have been implied earlier with a syringe and a gun but there was a problem with cost and some methods are very difficult that requires much care and expertise. These methods have been implemented not only for lungs but also for cerebral ventricles, bony labyrinth, vascular pattern of liver, kidney, spleen, coronary vessels etc. In present study Barbari goat lung was collected from postmortem cases in the department of Veterinary Pathology, ANDUAT, Kumarganj, Ayodhya. The fresh samples were utilized and silicone sealant was injected in trachea. The trachea was then gradually milked downwards as the silicone was forced into the lungs until the surface of the lungs appeared uneven. The apical bronchus and its segmental bronchi were observed in the caprine lung. This technique is easy, safe, economic and long lasting and can be used for teaching or for museum purposes.

Keywords

Tracheo-bronchial tree, silicon sealant and acid maceration

Conclusion

Corrosion casts of the airway of the lungs of mammals make excellent teaching tools and museum items. It looked great and was quite strong. The trachea and deflated lung of a Barbari goat were collected intact. Silicone resin that was sold commercially had a natural self-curing property. As a result, silicone resin was injected into the lung via the trachea. The resin deposited in the trachea must be manually pressed into the bronchi and its branches from the surface of the lungs since the resin's viscosity was thick and it cured quickly. It was known when the entire airway was filled with silicone resin when the normal colour of the surface of the lungs changed to the colour of the resin then the entire airway was filled with silicone resin. Then the specimen was hanged undisturbed for one night. Acid maceration may be done to obtain the cast quickly and the resulting cast may well be washed in water.

References

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How to cite this article

Nimgaonkar Mayur Sudhirrao, K.N. Singh, Mukesh Kumar, R.K. Joshi, Pankaj Kumar Choudhary, Sonu Jaiswal and Amit Singh Vishen (2022). Preparation of Low Cost Silicon Cast of Tracheo-Bronchial Tree of Barbari Goat. Biological Forum – An International Journal, 14(4): 453-455.