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Standardization of Surface Sterilization for in vitro Cloning of Pomegranate (Punica granatum L.) cv. Bhagwa

Author: Devendra Pal, Mukesh Kumar, Arvind Kumar, R.S. Sengar, M.K. Yadav, Anuj Pal, Kaushelendra Pratap Singh and Vibhu Pandey

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Abstract

Surface sterilization of explants in tissue culture determines the success or failure of any in vitro propagation system. In the present study, a number of sterilizing agents were used to minimize contamination and increase the survival percentage of pomegranate explants. Explants were alternately treated with fungicide (Tebuconzole 50% + Trifloxystorbin 25% w/w), mercuric chloride (0.1%), and 4% NaOCl2 in combination with each other for different time intervals, followed by ethanol treatment (70%) as the final disinfectant. Every combination gives differential results when they are applied for various durations. Maximum survival (73.33±11.55%) was recorded with the combination (4% NaOCl2 for 7 min + 0.1% HgCl2 for 2 min), followed by (66.67± 5.777%) and 63.33± 11.555%) survival recorded under the treatment (Tebuconzole 50% + Trifloxystorbin 25% w/w for 15 min + 0.1% HgCl2 for 2 min) and (4% NaOCl2 for 5 min + 0.1% HgCl2 for 1 min respectively. The combination of 4% NaOCl2 for 7 minutesn and 0.1% HgCl2 for 2minutesn was found to be the best among the combinations in the study, which gives the highest survival percentage in vitro propagation of pomegranate.

Keywords

Combination; HgCl2, NaOCl2 sterilization, tebuconzole, trifloxystorbin, pomegranate

Conclusion

In vitro propagation is the very sensitive system of plant propagation, which is positively correlated with the efficiency of sterilant. The exposure time of explants and different concentration of sterilizing agents may have a direct impact on the success rate as a result, the current investigation was carried out and resulted in the successful development of a complete sterilization package for the pomegranate.

References

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How to cite this article

Devendra Pal, Mukesh Kumar, Arvind Kumar, R.S. Sengar, M.K. Yadav, Anuj Pal, Kaushelendra Pratap Singh and Vibhu Pandey (2022). Standardization of Surface Sterilization for in Vitro Cloning of Pomegranate (Punica granatum L.) cv. Bhagwa. Biological Forum – An International Journal, 14(4): 653-656.