Isolation, Characterization, and Identification Candidate of Probiotic Rhodotorula mucilaginosa Isolated from Soaked and unsoaked Rice and Black gram Immersed in sterilized de-ionised Water

Author: Praveen Kumar Dubey, Ashish Rawson, Ananda Kumar Sugumar and N. Baskaran

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Abstract

In this investigation, soaked and unsoaked rice and black gram were submerged in sterile de-ionized water with the intention of examining the morphology, pigmentation, and identification of any microorganisms that may have grown there. Few studies have been done to determine the presence of particular probiotic bacteria in the soaked rice and black gram water. Over the potato dextrose agar (PDA) media, there were more than 234 yeast and mould colonies discovered. From these colonies, the most unique 4 colonies were chosen based on colour, characteristics, and gram staining, and from those, 2 colonies were used for PCR (Polymerase Chain Reaction) amplification. A single distinct 1050 bp PCR amplicon band was visible on an agarose gel. Through the use of NS1 and NS4 primer, the forward and reverse sequences of the 18S rRNA gene were combined to create the consensus sequence. However, Rhodotorula mucilaginosa's presence was validated by sequences that produced substantial alignments, a distance matrix, and a phylogenetic tree. The results of this investigation point to the confirmed the existence of Rhodotorula mucilaginosa in both soaked and unsoaked black gram.

Keywords

PCR amplification, agarose gel, Rhodotorula mucilaginosa, 18S rRNA

Conclusion

Findings of this study show that soaked and unsoaked rice and black gram submerged in sterile de-ionized water on the potato dextrose agar (PDA) media; there were more than 234 yeast and mould different colonies shown. From these colonies, the most identicle 4 colonies and from those 2 colonies were used in PCR amplification. A single species 1050 bp PCR amplicon band was visible on an agarose gel. The 18S rRNA gene's forward and reverse sequences were synthesized using the NS1 and NS4 primers to constructed the consensus sequence. However, Rhodotorula mucilaginosa's presence was validated by sequences that produced substantial alignments, a distance matrix, and a phylogenetic tree. Moreover, morphological and pigment also confirmed the availability of the Rhodotorula mucilaginosa in different water of black gram. Therefore, all the results of colour, characteristics, gram staining, and PCR amplification confirmed the existence of Rhodotorula mucilaginosa in both soaked and unsoaked black gram.

References

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How to cite this article

Praveen Kumar Dubey, Ashish Rawson, Ananda Kumar Sugumar, N. Baskaran (2022). Isolation, Characterization, and Identification Candidate of Probiotic Rhodotorula mucilaginosa Isolated from Soaked and unsoaked Rice and Black gram Immersed in sterilized de-ionised Water. Biological Forum – An International Journal, 14(4): 819-823.